Name: TINDALLIA TEXCOCOENSIS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1223 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	75.0	g	75	1283.37
Na₂CO₃	3.0	g	3	28.305
NaHCO₃	20.0	g	20	238.075
KCl	0.1	g	0.1	1.341
K₂HPO₄	0.3	g	0.3	1.722
MgCl₂ x 6 H₂O	0.5	g	0.5	2.459
CaCl₂ x 2 H₂O	0.2	g	0.2	1.36
NH₄Cl	1.0	g	1	18.695
Na₂S x 9 H₂O	0.4	g	0.4	1.665
Yeast extract	0.5	g	0.5	-
Peptone	5.0	g	5	-
Cysteine-HCl	0.5	g	0.5	3.172
Modified Wolin's mineral solution II	10.0	ml	-	-
Resazurin	10.0	mg	0.01	0.044
Distilled water	1000.0	ml	-	-
1 Adjust to pH 9.0
2 Prepare the medium omitting Na₂CO₃, NaHCO₃, Na₂S, Cysteine-HCl, CaCl₂ x 2 H₂O and MgCl₂ x 6 H₂O. Boil the medium and cool under nitrogen, add the Na₂CO₃ and NaHCO₃ and adjust the pH to 9.0 with 10 M KOH. Dispense into tubes or bottle fitted with rubber stoppers. Sterilize by autoclaving and add the Na₂S (neutralized), Cysteine-HCl, CaCl₂ x 2 H₂O and MgCl₂ x 6 H₂O from sterile stock solutions.

Modified Wolin's mineral solution II #

1000

Solution occurs in 24 other media (first defined in Medium 700).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.500	g	1.5	7.848
MgSO₄ x 7 H₂O	3.000	g	3	12.172
MnSO₄ x H₂O	0.500	g	0.5	2.958
NaCl	1.000	g	1	17.112
FeSO₄ x 7 H₂O	0.100	g	0.1	0.36
CoSO₄ x 7 H₂O	0.180	g	0.18	1.161
CaCl₂ x 2 H₂O	0.100	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.180	g	0.18	0.626
CuSO₄ x 5 H₂O	0.010	g	0.01	0.04
AlK(SO₄)₂ x 12 H₂O	0.020	g	0.02	0.042
H₃BO₃	0.010	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.010	g	0.01	0.041
NiCl₂ x 6 H₂O	0.025	g	0.025	0.193
Na₂SeO₃ x 5 H₂O	0.300	mg	3.0e-4	0.001
Distilled water	1000.000	ml	-	-
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

All strains for this medium:

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