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DSMZ

DSMZ 1231 . HALOPLASMA CONTRACTILE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1231 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 80.0 g 80 1368.93
KH2PO4 0.5 g 0.5 3.674
Yeast extract 0.2 g 0.2 -
Peptone 0.2 g 0.2 -
Starch 5.0 g 5 -
Na2S x 9 H2O 0.5 g 0.5 2.082
Resazurin 0.5 µg 5.0e-7 2.2e-6
Artificial seawater 250.0 ml - -
Distilled water 750.0 ml - -
1 Prepare the medium without the Na2S x 9 H2O by boiling and cooling under a nitrogen atmosphere. Adjust the pH to 6.5 - 7.0. Dispense into Hungate tubes or serum bottles fitted with butyl rubber stoppers. Autoclave and reduce the medium before use by adding the appropriate amount of Na2S x 9 H2O from a neutralised, sterile stock solution.

Artificial seawater #

1000
Equipment needed: Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.70 g 27.7 473.99
MgSO4 x 7 H2O 7.00 g 7 28.4
MgCl2 x 6 H2O 5.50 g 5.5 27.053
KCl 0.60 g 0.6 8.048
NaBr 0.10 g 0.1 0.972
H3BO3 0.03 g 0.03 0.485
CaCl2 x 2 H2O 1.50 g 1.5 10.203
SrCl2 x 6 H2O 15.00 mg 0.015 0.056
KI 0.05 mg 5.0e-5 1.9e-4
Double distilled water 1000.00 ml - -
1 Adjust pH to 5.5 - 6.0.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5 - 7.0
Equipment needed: Autoclave, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 6.925 118.497
Starch 5 -
MgSO4 x 7 H2O 1.75 7.1
MgCl2 x 6 H2O 1.375 6.763
KH2PO4 0.5 3.674
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain