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DSMZ

DSMZ 1343 . TISTLIA CONSOTENSIS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1343 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.30 g 0.3 1.722
KH2PO4 0.30 g 0.3 2.205
NaCl 20.00 g 20 342.231
NH4Cl 1.00 g 1 18.695
CaCl2 x 2 H2O 0.10 g 0.1 0.68
Yeast extract 1.00 g 1 -
KCl 0.10 g 0.1 1.341
Zeikus trace elements solution 1.00 ml - -
Distilled water 1000.00 ml - -
NaHCO3
(10 %) 		0.05 ml 0.005 0.06
Glucose 20.00 mM 3.603 20
Agar , for solid medium
optional 		15.00 g 15 -
1 Adjust pH to 7.0.
2 Autoclave at 121 °C for 15 min.
Glucose 20.00 mM 3.603 20

Zeikus trace elements solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.500 g 12.5 65.397
FeCl3 x 4 H2O 0.200 g 0.2 1.233
ZnCl2 0.100 g 0.1 0.734
MnCl2 x 4 H2O 0.100 g 0.1 0.505
H3BO3 0.010 g 0.01 0.162
CoCl2 x 6 H2O 0.017 g 0.017 0.131
CuCl2 x 2 H2O 0.020 g 0.02 0.117
CaCl2 x 2 H2O 0.100 g 0.1 0.68
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NaCl 1.000 g 1 17.112
Na2SeO3 0.020 g 0.02 0.116
Distilled water 1000.000 ml - -
1 Neutralise the nitrilotriacetic acid to a pH of 6.5 with KOH before adding the other trace elements.

Metadata

Last modified: 05.10.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		15 -
Glucose 3.603 20
NH4Cl 1 18.695
Yeast extract 1 -
KH2PO4 0.3 2.205
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain