1423
1423 .
METHYLOSINUS MEDIUM MM2
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1423 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KNO3 | 0.20 | g | 0.2 | 1.978 | |
| KH2PO4 | 0.04 | g | 0.04 | 0.294 | |
| MgSO4 x 7 H2O | 0.02 | g | 0.02 | 0.081 | |
| CaCl2 x 2 H2O | 4.00 | mg | 0.004 | 0.027 | |
| Trace elements Solution | 0.20 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 5.0. | |||||
| 2 Dispense the medium into serum bottles under gassing with CH4 (20% v/v). | |||||
| 3 Autoclave at 121 °C for 20 min. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 |
| CuCl2 x 5 H2O | 0.10 | g | 0.1 | 0.445 |
| FeSO4 x 7 H2O | 2.00 | g | 2 | 7.194 |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| H3BO3 | 0.30 | g | 0.3 | 4.851 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| Na2MoO4 | 0.03 | g | 0.03 | 0.146 |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 5.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 80 % Air
- 20 % CH4
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| KNO3 | 0.2 | 1.978 |
| KH2PO4 | 0.04 | 0.294 |
| MgSO4 x 7 H2O | 0.02 | 0.081 |
| CaCl2 x 2 H2O | 0.004 | 0.027 |
| EDTA | 1.0e-3 | 0.003 |
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