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DSMZ

DSMZ 1426 . SSE/HD 1:10 MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Granulicella mallensis DSM 23137

* Modification: Adjust pH of final medium to 5.5.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 20 °C

Main sol. 1426 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.50 g 0.5 -
Yeast extract 0.25 g 0.25 -
Glucose 0.10 g 0.1 0.555
MES
(10 mM final concentration) 		1.95 g 1.95 9.988
SSE (double concentrated) 500.00 ml - -
Distilled water 500.00 ml - -
1 Add to 1000 ml of medium after autoclaving:
Trace element solution SL-10 1.00 ml - -
Vitamin solution 1.00 ml - -
2 adjust pH to 5.5
3 For solid medium use purified agar (Oxoid LP0028) which has to be autoclaved separately.

SSE (double concentrated) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.2938 g 0.294 1.998
NH4Cl 0.1069 g 0.107 1.999
MgCl2 x 6 H2O 0.2036 g 0.204 1.001
(NH4)2SO4 0.1983 g 0.198 1.501
MgSO4 x 7 H2O 0.7390 g 0.739 2.998
CaSO4 x 2 H2O 0.8606 g 0.861 4.998
Ca(NO3)2 x 4 H2O 0.2360 g 0.236 0.999
Na NO3 0.4240 g 0.424 -
KH2PO4
(100 mM solution, 100 mM) 		0.5000 ml 0.007 0.05
FeSO4 x 7 H2O 0.0111 g 0.011 0.04
K2SO4 0.0870 g 0.087 0.499
Distilled water 1000.0000 ml - -
1 Dissolve by shaking overnight. Sterilize by autoclaving.

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 26.06.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 5.5
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Associated strains

All strains for this medium: 27
Strains with modifications: 16
Granulicella mallensis DSM 23137

  5.5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MES 1.95 9.988
Peptone 0.5 -
CaSO4 x 2 H2O 0.43 2.499
MgSO4 x 7 H2O 0.37 1.499
Yeast extract 0.25 -
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Cultivation metadata from 27 strains BacDive

halophily non-halophilic 3 strains
pH 6 - 8 14 strains
pH 4 - 6 12 strains
pH 8 - 10 10 strains
pH 2 - 4 7 strains
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