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DSMZ

DSMZ 1455 . ALTERNATIVE RUMEN BACTERIA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1455 #

ml
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.23 g 0.229 1.313
KH2PO4 0.23 g 0.229 1.68
NaCl 0.45 g 0.447 7.654
(NH4)2SO4 0.45 g 0.447 3.385
CaCl2 x 2 H2O 0.06 g 0.06 0.406
MgSO4 x 7 H2O 0.09 g 0.089 0.363
Brain heart infusion
(BD BBL) 		6.00 g 5.964 -
Yeast extract 5.00 g 4.97 -
Volatile fatty acid mixture 3.10 ml - -
Haemin solution
(0.05% w/v) 		2.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
NaHCO3 6.40 g 6.362 75.73
Disodium succinate 5.00 g 4.97 30.671
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.30 g 0.298 1.698
Na2S x 9 H2O 0.30 g 0.298 1.242
Distilled water 1000.00 ml - -
1 Dissolve ingredients except bicarbonate, succinate, vitamins, cysteine and sulfide, then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the bicarbonate and equilibrate the medium with the CO2 gas to reach a pH between 6.5 and 7.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add succinate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Prior to use adjust pH of complete medium to 6.6 - 6.8, if necessary.

Volatile fatty acid mixture #

1000
Solution occurs in 4 other media (first defined in Medium 330).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acetic acid 548.50 ml 573.182 9545.09
Propionic acid 193.50 ml 192.145 2593.76
Butyric acid 129.00 ml - -
n-Valeric acid 32.25 ml 30.283 296.512
iso-Butyric acid 32.25 ml 30.638 347.719
DL-2-Methylbutyric acid 32.25 ml - -
iso-Valeric acid 32.25 ml 30.025 293.986

Haemin solution #

100
Solution occurs in 15 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Haemin 50 mg 0.5 0.767
NaOH
(1 N) 		1 ml - -
Distilled water 100 ml - -
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 08.09.22
Source: DSMZ
Medium type: Complex medium
Final pH: 6.6 - 6.8
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 6.362 75.73
Brain heart infusion 5.964 -
Disodium succinate 4.97 30.671
Yeast extract 4.97 -
NaCl 0.447 7.654
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