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DSMZ 1456 . DESULFOSOMA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.3 g 0.3 1.722
KH2PO4 0.3 g 0.3 2.205
NaCl 1.0 g 1 17.112
NH4Cl 1.0 g 1 18.695
KCl 0.1 g 0.1 1.341
CaCl2 x 2 H2O 0.1 g 0.1 0.68
MgCl2 x 6 H2O 0.5 g 0.5 2.459
Na2SO4 2.8 g 2.8 19.713
Yeast extract
(OXOID)
1.0 g 1 -
Trypticase peptone
(BD BBL)
1.0 g 1 -
Trace element solution SL-10 1.0 ml - -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
Na2CO3 1.5 g 1.5 14.153
Na-pyruvate 2.5 g 2.5 22.718
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
Na2S x 9 H2O 0.5 g 0.5 2.082
Distilled water 1000.0 ml - -
1 Dissolve ingredients except carbonate, pyruvate, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 minto make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary.
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes

Gas composition

  • 20 % CO2
  • 80 % N2

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.8 19.713
Na-pyruvate 2.5 22.718
Na2CO3 1.5 14.153
NH4Cl 1 18.695
NaCl 1 17.112
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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