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DSMZ

DSMZ 1488 . METHYLOCEANUS CENITEPIDUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1488 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 (10x NMS salts) 100.0 ml - -
NaCl
(2 %, in distilled water) 		900.0 ml 18 308.008
Solution 2 (Fe EDTA) 0.1 ml - -
Solution 3 (Sodium molybdate) 1.0 ml - -
Trace elements 1.0 ml - -
Agar , for solid medium 1.5 % 15 44.599
Phosphate buffer 10.0 ml - -
Methanol 1.0 % 10 312.09
1 Dilute 100 ml solution 1 to 1 litre with 2% NaCl and then add 1 ml of solution 3, 1 ml of the trace elements, and 0.1 ml of solution 2. If solid media is required add 1.5% agar. Autoclave the phosphate buffer separately. When the growth medium is cool 10 ml/l of the phosphate buffer is added - if too warm the phosphate will precipitate.
2 Add filter-sterilized methanol to produce 1% (final concentration).

Trace elements #

100
Solution occurs in 1 other media (first defined in Medium 1488).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 25.0 mg 0.25 0.744
CuSO4 x 5 H2O 20.0 mg 0.2 0.801
FeSO4 x 7 H2O 50.0 mg 0.5 1.798
ZnSO4 x 7 H2O 40.0 mg 0.4 1.391
H3BO3 1.5 mg 0.015 0.243
CoCl2 x 6 H2O 5.0 mg 0.05 0.385
MnCl2 x 4 H2O 2.0 mg 0.02 0.101
NiCl2 x 6 H2O 1.0 mg 0.01 0.077
Distilled water 100.0 ml - -
1 May be stored at 4°C in the dark

Phosphate buffer #

1000
Solution occurs in 2 other media (first defined in Medium 921).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 71.6 g 71.6 504.371
KH2PO4 26.0 g 26 191.057
Distilled water 1000.0 ml - -
1 Dissolve in about 800 ml of water, adjust the pH to 6.8 and make up to 1 litre.

Solution 1 (10x NMS salts) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 10 g 10 186.951
MgSO4 x 6 H2O 10 g 10 83.078
CaCl2 x 2 H2O 2 g 2 13.604
NaCl
(2%, in distilled water) 		1000 ml 20 342.231
1 Dissolve the ingredients listed above (in that order) in about 700 ml of 2% NaCl, and then make up to 1 litre with 2% NaCl.

Solution 2 (Fe EDTA) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(III)-EDTA 3.8 g 38 110.447
Distilled water 100.0 ml - -

Solution 3 (Sodium molybdate) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2MoO4 x 2 H2O 0.26 g 0.26 1.075
Distilled water 1000.00 ml - -

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 18 308.008
Agar 15 44.599
Methanol 10 312.09
NH4Cl 1 18.695
MgSO4 x 6 H2O 1 8.308
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain