DSMZ 1560 . MARINES PLANCTOMYCES MEDIUM

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Culture medium recipe

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Selected strain: Aeoliella mucimassa DSM 29370

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone
(Bacto)
0.25 g 0.25 -
Yeast extract
(Bacto)
0.25 g 0.25 -
HEPES 2.38 g 2.38 9.987
Mineral salts solution 20.00 ml - -
Trace element solution 1.00 ml - -
Artifical sea water 250.00 ml - -
Agar
(Bacto)
15.00 g 15 44.599
Double distilled water 730.00 ml - -
1 Sterilize 20 min./121°C. After cooling to 60°C add:
N-Acetylglucosamine
(10% stock solution, 10 % stock solution)
8.00 ml 0.8 3.616
Glucose
(2.5%, sterile-filtered)
10.00 ml 0.25 1.388
Vitamin solution (double conc.)
(medium 621)
5.00 ml - -
2 Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).
Solution occurs in 2 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 46.940 g 46.94 803.217
Na2SO4 7.840 g 7.84 55.196
MgCl2 x 6 H2O 21.280 g 21.28 104.671
CaCl2 2.860 g 2.86 25.77
NaHCO3 0.380 g 0.38 4.523
KCl 1.380 g 1.38 18.511
KBr 0.192 g 0.192 1.613
H3BO3 0.052 g 0.052 0.841
SrCl2 0.080 g 0.08 0.505
NaF 6.000 mg 0.006 0.143
Double distilled water 1000.000 ml - -
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.00 g 10 52.318
MgSO4 x 7 H2O 29.70 g 29.7 120.499
CaCl2 x 2 H2O 3.34 g 3.34 22.719
Na2MoO7O4 x 2 H2O 12.67 mg 0.013 -
FeSO4 x 7 H2O 99.00 mg 0.099 0.356
"Metals 44" 50.00 ml - -
Distilled water 950.00 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-Nitrilotriacetat 1.50 g 1.5 -
MnSO4 x H2O 0.50 g 0.5 2.958
Fe SO4 x 7 H2O 0.10 g 0.1 0.36
Co(NO3)2 x 6 H2O 0.10 g 0.1 0.344
ZnCl2 0.10 g 0.1 0.734
NiCl2 x 6 H2O 0.05 g 0.05 0.386
H2SeO3 0.05 g 0.05 0.388
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.01 g 0.01 0.021
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Na2WO4 x 2 H2O 0.01 g 0.01 0.03
Double distilled water 1000.00 ml - -
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.0 mg 0.004 0.016
Folic acid 4.0 mg 0.004 0.009
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Riboflavine 10.0 mg 0.01 0.027
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
D-Calcium pantothenate 10.0 mg 0.01 -
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Double distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
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Associated strains

All strains for this medium: 58
Strains with modifications: 1
Aeoliella mucimassa DSM 29370

No modifications necessary for this strain.

Media similar to medium 1560

1560a PLANCTOMYCES MEDIUM WITH DOUBLE CONCENTRATED ARTIFICAL SEA WATER
1695 MARINES PLANCTOMYCES MEDIUM MODIFIED I

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
NaCl 11.735 200.804
MgCl2 x 6 H2O 5.32 26.168
HEPES 2.38 9.987
Na2SO4 1.96 13.799
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Cultivation metadata from 58 strains BacDive

pH 6 - 8 32 strains
pH 8 - 10 29 strains
pH 4 - 6 12 strains
phyla 2 46 strains
nutrition chemoheterotroph 1 strain
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