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DSMZ

DSMZ 1598 . VERRUCOMICROBIUM CANDIDATUS MEDIUM (KAM1)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1598 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
10x NMS salts 100 ml - -
Distilled water 900 ml - -
FeNaEDTA solution 4.5 g/L 1 ml - -
Trace Element Solution 1 ml - -
Phosphate buffer 20 ml - -
1 Dilute 100 ml NMS salt solution to 1 litre with distilled water and then add 1 ml of FeNaEDTA solution and 1 ml of the trace elements and autoclave. Also autoclave the phosphate buffer stock solution.
2 After cooling of the autoclaved solutions, add 20ml phosphate buffer stock solution to the mineral solution.
3 Dispense 9ml distilled water into the growth vessels. Use sealed vessels it is appropriate to add 10% methane and 1% CO2 gas to the gas phase and autoclave.
4 When the growth vessels is cool, 1.0 ml of the mineral medium and 0.1 ml 0.03 M Cerium-(III)-chlorid are added.
5 Adjust pH to 3.5 with 1M HCL to obtain the final mineral medium before inoculate.
6 The cultures should be grown with shaking.

10x NMS salts #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 10 g 10 186.951
MgSO4 x 7 H2O 10 g 10 40.572
CaCl2 x 2 H2O 2 g 2 13.604
Distilled water 1000 ml - -
1 Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre

Trace Element Solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.25 g 0.25 0.744
ZnSO4 x 7 H2O 0.40 g 0.4 1.391
MnCl2 x 4 H2O 0.02 g 0.02 0.101
H3BO3 0.02 g 0.02 0.323
Na2MoO4 x 2 H2O 0.04 g 0.04 0.165
NiCl2 x 2 H2O 0.01 g 0.01 0.077
CuSO4 x 5 H2O 0.20 g 0.2 0.801
CoCl2 x 6 H2O 0.05 g 0.05 0.385
Distilled water 1000.00 ml - -
1 May be stored at 4°C in the dark

Phosphate buffer #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 37.325 g 37.325 274.277
Na2HPO4 x 12 H2O 48.950 g 48.95 344.818
Distilled water 1000.000 ml - -

FeNaEDTA solution 4.5 g/L #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeNa-EDTA 4.5 g 4.5 -
Distilled water 1000.0 ml - -

Cerium(III)chloride: (0.03M) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
CeCl3 x 7 H2O 11.2 g 11.2 45.441
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: 3.5
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NH4Cl 0.978 18.293
MgSO4 x 7 H2O 0.978 3.97
Na2HPO4 x 12 H2O 0.958 6.748
KH2PO4 0.73 5.367
CaCl2 x 2 H2O 0.196 1.331
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