DSMZ 1635 . SULFURIFLEXUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.0 g 20 342.231
MgCl2 x 6 H2O 3.0 g 3 14.756
MgSO4 x 7 H2O 0.3 g 0.3 1.217
CaCl2 0.1 g 0.1 0.901
NH4Cl 0.1 g 0.1 1.87
KH2PO4 0.1 g 0.1 0.735
KCl 0.1 g 0.1 1.341
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Na2S2O3 x 5 H2O 5.0 g 5 20.146
Wolin's vitamin solution 10.0 ml - -
NaHCO3 0.4 g 0.4 4.762
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except thiosulfate, bicarbonate and vitamins), adjust pH to 6.0 and dispense medium under air atmosphere into serum vials up to a volume of 20%, close with rubber septa and autoclave. Add thiosulfate, vitamins and bicarbonate to the autoclaved medium from sterile stock solutions sterilized by filtration. Adjust pH of the complete medium to 7.2.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 12.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Serum bottle
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Gas composition

  • 100 % Air

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
Na2S2O3 x 5 H2O 5 20.146
MgCl2 x 6 H2O 3 14.756
NaHCO3 0.4 4.762
MgSO4 x 7 H2O 0.3 1.217
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
nutrition autotroph 1 strain
nutrition chemolithoautotroph 1 strain
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