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1635. SULFURIFLEXUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.0 g 19.763 338.159
2 3.0 g 2.964 14.581
3 0.3 g 0.296 1.203
4 0.1 g 0.099 0.89
5 0.1 g 0.099 1.847
6 0.1 g 0.099 0.726
7 0.1 g 0.099 1.325
8 Trace element solution SL-10 1.0 ml - -
9 Selenite-tungstate solution 1.0 ml - -
10 5.0 g 4.941 19.907
11 Vitamin solution 10.0 ml - -
12 0.4 g 0.395 4.705
13 1000.0 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients (except thiosulfate, bicarbonate and vitamins), adjust pH to 6.0 and dispense medium under air atmosphere into serum vials up to a volume of 20%, close with rubber septa and autoclave. Add thiosulfate, vitamins and bicarbonate to the autoclaved medium from sterile stock solutions sterilized by filtration. Adjust pH of the complete medium to 7.2.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.198 55408.3
NaCl 19.763 338.159
Na2S2O3 x 5 H2O 4.941 19.907
MgCl2 x 6 H2O 2.964 14.581
NaHCO3 0.395 4.705
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Cultivation metadata from 2 strains BacDive

halophily not 1 strain
halophily slightly 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
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