MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1637 . METHANOMASSILIICOCCUS LUMINYENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1637 #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone
(BD BACTO) 		16.0 g 15.686 -
Yeast extract
(OXOID) 		7.0 g 6.863 -
NaCl 5.0 g 4.902 83.88
L-Cysteine HCl x H2O 0.5 g 0.49 2.791
NaHCO3 0.5 g 0.49 5.835
Haemin solution 5.0 ml - -
Vitamin K1 solution 5.0 ml - -
Sodium resazurin
(0.1% w/v) 		0.5 ml 4.9e-4 0.002
Methanol
(15% v/v) 		10.0 ml 7.765 242.329
DL-Dithiothreitol 1.0 g 0.98 6.354
Distilled water 1000.0 ml - -
1 Dissolve ingredients except for methanol and dithiothreitol. Adjust pH to 7.5 with 1 N NaOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add methanol from a sterile anoxic stock solution (15% v/v) autoclaved under 100% N2 gas atmosphere and dithiothreitol from an anoxic stock solution sterilized by filtration. Before use check the pH of the complete medium and adjust it to 7.6 - 7.8, if necessary.
2 After inoculation, add sterile 80% H2 and 20% CO2 gas mixture to 0.5 bar overpressure. After growth becomes visible overpressure of 80% H2 and 20% CO2 gas mixture can be increased to 1 bar.
3 Note: Use 10% (v/v) as inoculum.

Haemin solution #

100
Solution occurs in 15 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Haemin 50 mg 0.5 0.767
NaOH
(1 N) 		1 ml - -
Distilled water 100 ml - -
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Vitamin K1 solution #

20
Solution occurs in 12 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin K1 0.1 ml - -
Ethanol
(95 %) 		20.0 ml 950 20620.8
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Metadata

Last modified: 13.12.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.6 - 7.8
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
Edit in Medium builder

Gas composition

  • 66 % N2
  • 27 % H2
  • 7 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 15.686 -
Methanol 7.765 242.329
Yeast extract 6.863 -
NaCl 4.902 83.88
Ethanol 4.657 101.082
Show more
CSV
JSON