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DSMZ

DSMZ 1683 . CM1A Halorubrum Medium

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1683 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
30% Salt Water (1L)
(30%) 		829.0 ml - -
23% MGM (100mL)
(23%) 		5.0 ml - -
Sodium pyruvate 5.5 g 5.5 49.982
Distilled water 155.0 ml - -
1 Adjust pH to 7.5 with 1 M HCl or 1 M NaOH
2 Autoclave at 121 °C for 20 min.
3 Before inoculation add from sterile stock solutions:
NH4Cl
(1M) 		5.0 ml 0.267 5
K2HPO4
(0.5M) 		2.0 ml 0.174 1
Trace element solution SL-10 1.0 ml - -
Vit 10 3.0 ml - -

30% Salt Water (1L) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240.00 g 240 4106.78
MgCl2 x 6 H2O 30.00 g 30 147.563
MgSO4 x 7 H2O 35.00 g 35 142.002
CaCl2 x H2O 0.74 g 0.74 6.668
Distilled water 1000.00 ml - -
1 Dissolve salts completely then add CaCl2 x H2O.
2 Adjust pH to 7.5 with Tris-HCl at pH=7.5

23% MGM (100mL) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Salt water
(30%) 		76.6 ml - -
Peptone
(VWR J636-500g) 		0.5 g 5 -
Yeast extract
(Fisher BioReagents BP1422-500) 		0.1 g 1 -
Distilled water 23.4 ml - -
1 Don't use Difco Bacto-peptone, it was reported in 1988 to contain bile salts that lyse halobacteria, this was still the case in 2001
2 Adjust pH to 7.5

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Vitamin solution 1 = Vit 1 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 40 mg 0.04 0.292
Biotin 10 mg 0.01 0.041
Nicotinic acid 100 mg 0.1 0.812
Calcium D-(+)-pantothenate 50 mg 0.05 -
Pyridoxamine hydrochloride 150 mg 0.15 0.733
Thiamine hydrochloride 100 mg 0.1 0.296
Cyanocobalamin
(B12) 		50 mg 0.05 0.037
1 filter sterilized

Vitamin solution 2 = Vit 2 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
DL-6,8-thioctic acid 10 mg 0.01 0.048
Riboflavin 10 mg 0.01 0.027
Folic acid 4 mg 0.004 0.009
1 filter sterilized

Vit 10 #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin solution 1 = Vit 1 0.25 ml - -
Vitamin solution 2 = Vit 2 0.75 ml - -
1 mix 0.25 ml Vit 1 and 0.75 ml Vit2

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 198.96 3404.52
MgSO4 x 7 H2O 29.015 117.72
MgCl2 x 6 H2O 24.87 122.33
Sodium pyruvate 5.5 49.982
CaCl2 x H2O 0.613 5.528
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