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DSMZ

DSMZ 1712 . AROMATOLEUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Aromatoleum aromaticum DSM 19016

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 1712 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.5 g 0.526 3.868
NH4Cl 0.3 g 0.316 5.904
MgSO4 x 7 H2O 0.5 g 0.526 2.135
CaCl2 x 2 H2O 0.1 g 0.105 0.716
NaNO3 0.6 g 0.632 7.431
Distilled water 950.0 mL - -
1 The medium was autoclaved and removed from the autoclave at 85°C. Oxygen dissolution was prevented by exchanging the gas-phase with N2. The medium was complemented by addition of the following stock solutions:
Trace element solution 1.0 mL/L - -
Vitamin mixture 1.0 mL/L - -
Vitamin B12 solution 1.0 mL/L - -
Bicarbonate solution (1M) 40.0 mL/L 3.36 40
2 After adding bicarbonate the gas was changed from N2 to N2/CO2 (90/10 %, v/v) and the pH was adjusted to 7.2 - 7.4 with sterile 2 M HCL.
Ascorbate solution (1M) 4.0 mL/L - 4
Benzoate solution (1M) 4.0 mL/L 0.484 4

Trace element solution #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.200 g 5.2 15.467
FeSO4 x 7 H2O 2.100 g 2.1 7.554
H3BO3 0.030 g 0.03 0.485
MnCl2 x 4 H2O 0.100 g 0.1 0.505
CoCl2 x 6 H2O 0.190 g 0.19 1.463
NiCl2 x 6 H2O 0.024 g 0.024 0.185
CuSO4 x 5 H2O 0.029 g 0.029 0.116
ZnSO4 x 7 H2O 0.144 g 0.144 0.501
Na2MoO4 x 2 H2O 0.036 g 0.036 0.149
1 The trace element solution was prepared by dissolving the salts in 800 mL warm H2Obidest
2 NaOH was used to adjust the pH to 6.5. The solution was added up to 1000 mL with H2Obidest and autoclaved.

Vitamin mixture #

100
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4-NaH2PO4 buffer , 25 mM, pH 7.1 100.0 mL 1820 -
4-Aminobenzoic Acid 4.0 mg 0.04 0.292
D-(+)-biotin 1.0 mg 0.01 0.041
Nicotinic acid 10.0 mg 0.1 0.812
Calcium D-(+)-pantothenate 5.0 mg 0.05 -
Pyridoxal hydrochloride 15.0 mg 0.15 0.737
DL-alpha-lipoic acid 1.5 mg 0.015 0.073
Folic acid 4.0 mg 0.04 0.091
2-Mercaptoethanesulfonic acid , sodium salt 25.0 mg 0.25 1.758
1 The solution was filter-sterilized and stored at 4°C in the dark.

Vitamin B12 solution #

50
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine 2.5 mg 0.05 0.037
Double distilled water 50.0 mL - -
1 The solution was filter-sterilized and stored at 4°C in the dark.

Bicarbonate solution (1M) #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 84 g 84 999.917
Double distilled water 1000 mL - -
1 The solution was dispensed in the required portions (40 mL per 1 L medium). The head space of the bottles was flushed with CO2. Saturation of CO2 in the solution was achieved by repeated flushing and shaking of the bottles. Finally, the bottles were sealed with rubber stoppers and aluminum crimps and autoclaved.

Ascorbate solution (1M) #

100
Equipment needed: Filter Autoclave Ice bath
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ascorbic acid 17.6 g 176 999.319
Double distilled water 100.0 mL - -
1 In an ice bath ascorbic acid was dissolved in 80 mL anoxic H2Obidest (autoclaved, cooled under N2 atmosphere) while flushing with N2. The pH was adjusted to 7.0 with NaOH and the solution added up to 100 ml with anoxic H2Obidest. After filter-sterilization the solution was stored under N2 atmosphere in a special flask (which can be flushed with gas) at 4°C in the dark.

Benzoate solution (1M) #

100
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodiumbenzoate 14.4 g 144 -
Double distilled water 100.0 mL - -
1 The solution was filter-sterilized and stored at room temperature.

Metadata

Last modified: 03.01.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter, Ice bath
Description:

Based on the medium in Rabus and Widdel, 1995, Arch. Microbiol., 163, 96-103.
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Associated strains

All strains for this medium: 5
Aromatoleum aromaticum DSM 19016

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.537 42.102
Na2HPO4-NaH2PO4 buffer 1.916 -
Ascorbic acid 0.741 4.208
NaNO3 0.632 7.431
Sodiumbenzoate 0.606 -
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Cultivation metadata from 5 strains BacDive

phyla 1 5 strains
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 5 strains