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1721. MMJHS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 1 11.765
2 1 g 1 11.904
3 1 g 1 4.029
4
(powdered)
10 g 10 311.857
5 Synthetic seawater 1000 ml - -
Instructions:
  1. Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding NaNO3, Na2S2O3 x 5 H20 and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, NaNO3, Na2S2O3 x 5 H20 and NaHCO3.
  2. Increase the 80% H2 + 20% CO2 gas phase pressure to 0.3 MPa. The final pH is 7.0.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 25.00 g 24.753 423.536
2 0.14 g 0.139 0.796
3 0.70 g 0.693 4.714
4 0.25 g 0.248 4.627
5 3.40 g 3.366 13.658
6 4.20 g 4.158 20.454
7 0.50 g 0.495 6.64
8 Trace mineral solution 10.00 ml - -
9 1000.00 ml 988.324 54860.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.500 g 1.5 7.848
1 3.000 g 3 12.172
10 0.500 g 0.5 2.958
11 1.000 g 1 17.111
12 0.100 g 0.1 0.36
13 0.180 g 0.18 0.64
14 0.100 g 0.1 0.68
15 0.180 g 0.18 0.626
16 0.010 g 0.01 0.04
17 0.002 g 0.002 0.008
18 0.010 g 0.01 0.162
19 0.010 g 0.01 0.041
20 0.100 g 0.1 0.303
21 0.025 g 0.025 0.105
22 0.050 g 0.05 0.19
23 1000.000 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Filter

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 24.762 423.705
Sulfur 10 311.857
MgCl2 x 6 H2O 4.158 20.454
MgSO4 x 7 H2O 3.396 13.778
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