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202. DESULFONEMA MAGNUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 1000 ml - -
2 Solution B 10 ml - -
3 Solution C 10 ml - -
4 Solution D 10 ml - -
5 Solution E 20 ml - -
6 Solution F 10 ml - -
7 , may stimulate growth of some strains
(5% w/v)
20 mg/l 0.02 -
Instructions:
  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to reach a pH of around 6, then distributed under the same gas atmosphere into anoxic Hungate-type tubes and autoclaved. Solutions B, D, and F are autoclaved separately under 100% N2 gas. Solution E is autoclaved under 80% N2 and 20% CO2 gas mixture. Solution C is prepared under 100% N2 gas and sterilized by filtration.
  2. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0. After completion the medium should equilibrate overnight and a white precipitate should be apparent.
  3. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution freshly prepared under N2 and filter-sterilized) just before inoculation may stimulate growth at the beginning. For transfers use 5 - 10% inoculum.

Solution A #

1002
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 25.00 g 24.95 426.917
2 5.60 g 5.589 27.49
3 6.80 g 6.786 27.534
4 1.40 g 1.397 9.504
5 0.72 g 0.719 9.639
6 0.14 g 0.14 1.027
7 0.25 g 0.25 4.664
8 Trace element solution SL-10 1.00 ml - -
9 Selenite-tungstate solution 1.00 ml - -
10
(0.1% w/v)
0.50 ml 5.0e-4 0.002
11 1000.00 ml 996.215 55298.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.6 g 60 416.369
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.48 g 48 101.183
2 10.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 50 471.752
2 20 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 124.905
2 10.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.198 55408.3
NaCl 23.538 402.752
MgSO4 x 7 H2O 6.402 25.975
MgCl2 x 6 H2O 5.272 25.934
CaCl2 x 2 H2O 1.318 8.966
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