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DSMZ

DSMZ 204 . ASPERGILLUS NIDULANS MINIMAL MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 204 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 250 ml - -
Solution C 200 ml - -
1 Autoclave separately for 15 min. Mix A, B and C before pouring plates.

Solution A #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 6.00 g 12 141.185
KH2PO4 1.52 g 3.04 22.339
KCl 1.52 g 3.04 40.778
Distilled water 500.00 ml - -
1 Adjust pH to 6.5 with 2 N NaOH.

Solution B #

250
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.52 g 2.08 8.439
FeSO4 x 7 H2O trace - -
ZnSO4 x 7 H2O trace - -
Agar 15.00 g 60 178.396
Distilled water 250.00 ml - -

Solution C #

200
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 10 g 50 277.531
Distilled water 200 ml - -

Metadata

Last modified: 18.05.22
Source: DSMZ
Taxonomic range:  Fungi
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15.79 46.946
Glucose 10.526 58.428
NaNO3 6.316 74.308
KCl 1.6 21.462
KH2PO4 1.6 11.757
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