215c
215c .
BHI MEDIUM FOR STRICT ANAEROBES
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Roseburia porci DSM 107448
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
aerobic
37 °C
pH 7.00
Main sol. 215c #
ml
Equipment needed: Gassing station
Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Brain heart infusion
(DIFCO) |
37.00 | g | 37 | - | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | 1.423 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2. | |||||
Metadata
| Last modified: | 18.01.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 - 7.6 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 7 |
| Strains with modifications: | 3 |
Roseburia porci DSM 107448
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Brain heart infusion | 37 | - |
| L-Cysteine HCl x H2O | 0.25 | 1.423 |
| Na2S x 9 H2O | 0.25 | 1.041 |
| Distilled water | - | - |
