249
249 .
FAECALICATENA OROTICA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 249 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Tryptone
(BD BACTO) |
5.00 | g | 5 | 70.343 | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| KH2PO4 | 1.36 | g | 1.36 | 9.994 | |
| K2HPO4 | 6.95 | g | 6.95 | 39.902 | |
| Na-orotate | 2.50 | g | 2.5 | 14.039 | |
| Riboflavin | 15.00 | mg | 0.015 | 0.04 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Na-thioglycolate | 0.50 | g | 0.5 | 4.382 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients and adjust pH to 7.5. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. | |||||
Metadata
| Last modified: | 08.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| K2HPO4 | 6.95 | 39.902 |
| Tryptone | 5 | 70.343 |
| Na-orotate | 2.5 | 14.039 |
| KH2PO4 | 1.36 | 9.994 |
| Na-thioglycolate | 0.5 | 4.382 |
| Show more | ||
