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DSMZ

DSMZ 253 . MEDIUM FOR ECTOTHIORHODOSPIRA

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 253 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.495 3.634
CaCl2 x 2 H2O 0.05 g 0.049 0.336
NH4Cl 0.80 g 0.791 14.793
MgCl2 x 6 H2O 0.10 g 0.099 0.487
NaCl 180.00 g 178.042 3046.57
Na2SO4 20.00 g 19.782 139.273
Na2CO3 6.00 g 5.935 55.994
Trace element solution (A) 1.00 ml - -
Yeast extract 0.50 g 0.495 -
Sodium succinate 1.00 g 0.989 6.104
Distilled water 800.00 ml - -
1 Adjust pH to 8.5.
2 Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. After sterilization add 1 ml/l filter-sterilized vitamin solution (B), 200 ml of filter-sterilized solution Bicarbonate solution (C) and 10 ml/l sterilised Na2S x 9H2O solution (D). pH 8.5
Vitamin solution (B) 1.00 ml - -
Bicarbonate solution (C) 200.00 ml - -
Na2S x 9H2O solution (D) 10.00 ml - -

Trace element solution (A) #

1000
Solution occurs in 1 other media (first defined in Medium 253).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 1.8 g 1.8 9.054
CoCl2 x 6 H2O 250.0 mg 0.25 1.925
NiCl2 x 6 H2O 10.0 mg 0.01 0.077
CuCl2 x 2 H2O 10.0 mg 0.01 0.059
MnCl2 x 4 H2O 70.0 mg 0.07 0.354
ZnCl2 100.0 mg 0.1 0.734
H3BO3 500.0 mg 0.5 8.085
Na2MoO4 x 2 H2O 30.0 mg 0.03 0.124
Na2SeO3 x 5 H2O 10.0 mg 0.01 0.038
Distilled water 1000.0 ml - -
1 For dissolving adjust pH to about 3 with 1 N HCl.

Vitamin solution (B) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 10 mg 0.1 0.409
Nicotinamide 35 mg 0.35 2.866
Thiamine-HCl x 2 H2O 30 mg 0.3 0.804
p-Aminobenzoic acid 20 mg 0.2 1.458
Pyridoxal hydrochloride 10 mg 0.1 0.491
Calcium pantothenate 10 mg 0.1 0.21
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Bicarbonate solution (C) #

200
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 14 g 70 833.264
Distilled water 200 ml - -

Na2S x 9H2O solution (D) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 10 g 100 416.351
Distilled water 100 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 178.042 3046.57
Na2SO4 19.782 139.273
NaHCO3 13.848 164.84
Na2CO3 5.935 55.994
Sodium succinate 0.989 6.104
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Cultivation metadata from 5 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature mesophilic 3 strains
temperature thermophilic 1 strain