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DSMZ

DSMZ 298 . PELOBACTER PROPIONICUS MEDIUM (FRESHWATER)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Salileptolyngbya sp. DSM 8909

* Modification: Supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas).

Growth conditions: anaerobic 30 °C

Main sol. 298 #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.2 1.47
NH4Cl 0.25 g 0.25 4.674
NaCl 1.00 g 1 17.112
MgCl2 x 6 H2O 0.40 g 0.4 1.968
KCl 0.50 g 0.5 6.707
CaCl2 x 2 H2O 0.15 g 0.15 1.02
Trace element solution SL-10 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Na2CO3 1.50 g 1.5 14.153
2,3-butanediol* 0.90 g 0.9 9.987
Na2S x 9 H2O 0.36 g 0.36 1.499
Triethanolamine hydrochloride* 1.40 g - -
Seven vitamins solution* 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients except carbonate, 2,3-butanediol and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.2 - 7.5.
2 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Seven vitamins solution * #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 31.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2 - 7.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 12
Strains with modifications: 11
Salileptolyngbya sp. DSM 8909

  1.4 g Triethanolamine hydrochloride
+  1 ml Seven vitamins solution
  2,3-butanediol

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 1.5 14.153
NaCl 1 17.112
2,3-butanediol 0.9 9.987
KCl 0.5 6.707
MgCl2 x 6 H2O 0.4 1.968
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Cultivation metadata from 12 strains BacDive

phyla 3 12 strains
oxygen anaerobe 12 strains
temperature mesophilic 12 strains