Loading ...

336. OXOBACTER PFENNIGII MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 970 ml - -
2 Solution B 30 ml - -
3 Solution C 1 ml - -
4 Solution D 10 ml - -
5 Solution E 3 ml - -
Instructions:
  1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, distribute under same gas atmosphere 8.9 ml medium per Hungate-type tube and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere and sterilized by filtration. Autoclave solutions D and E under 100% N2 gas. To complete the medium add appropriate amounts of the solutions B to E to the sterile solution A. Adjust pH of complete medium to 7.0 - 7.2, if necessary.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Mineral solution 50.0 ml - -
2 Trace element solution SL-10 1.0 ml - -
3 2.0 g 2.058 -
4 2.0 g 2.058 10.822
5 Clarified rumen fluid 300.0 ml - -
6
(0.1% w/v)
0.5 ml 5.1e-4 0.002
7 620.0 ml 636.716 35343.1
Instructions:
  1. Adjust pH to 6.9 before autoclaving.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Seven vitamins solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 170.808
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 90 mg 30 124.905
2 3 ml 998.207 55408.8
Solution occurs in 3 other media (first defined in medium 335 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.0 g 10 73.484
2 6.6 g 6.6 32.464
3 8.0 g 8 136.887
4 8.0 g 8 149.557
5 1.0 g 1 6.802
6 1000.0 ml 998.207 55408.8
Solution occurs in 32 other media (first defined in medium 1310 )
Equipment needed: Centrifuge Filter Autoclave
Instructions:
  1. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 minand then centrifuged at 27,000 g for 20 min The supernatant is made anoxic by sparging with 100% N2 gas for 15 min dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at - 20°C.
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Centrifuge, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 702.495 38994.4
Na-vanillate 1.968 10.353
Yeast extract 1.968 -
Na2CO3 1.479 13.957
KH2PO4 0.492 3.616
Show more

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

Similar media

900. ACETOBACTERIUM TUNDRAE MEDIUM 87 %
298a. LUPHET1 MEDIUM 87 %
1667. HYPNOCYCLICUS MEDIUM 86 %
788. CALORAMATOR PROTEOCLASTICUS MEDIUM 86 %
875. DESULFACINUM HYDROTHERMALE MEDIUM 85 %

Latest comments

No comments for this medium.