Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Supplement medium after autoclaving with 0.2 g/l dithiothreitol (DTT) added from a filter-sterilized anoxic stock solution and adjust pH of complete medium to 7.2.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
dehydrated Wilkins-Chalgren medium
|1 Dissolve 33.0 g/l dehydrated Wilkins-Chalgren medium (Oxoid CM0643) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving supplement medium with 5.0 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.8 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.|
|2 Note: In case resazurin is not colourless addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) can be used to completely reduce the medium.|
|Medium type:||Complex medium|
|Final pH:||* 7.2|
|Equipment needed:||Autoclave, Filter, Gassing station, Hungate tubes|
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
|All strains for this medium:||35|
|Strains with modifications:||8|
|Compound||Conc. [g/L]||Conc. [mM]|
|dehydrated Wilkins-Chalgren medium||33||-|