Culture medium recipe
Equipment needed: Hungate tubes Filter Autoclave
|#||Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
Dehydrated Wilkins-Chalgren medium
|4||L-Cysteine HCl x H2O||0.3||g/l||0.3||1.708|
, see note
- Dissolve 33.0 g/l dehydrated Wilkins-Chalgren medium (Oxoid CM0643) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine- HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8 - 7.0, if necessary.
- Note: In case resazurin is not colourless addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) can be used to completely reduce the medium.