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402. ALICYCLOBACILLUS MEDIUM

Culture medium recipe

Vol.
×
  • For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 1000 ml - -
2 Solution B 1 ml - -
3 Solution C 500 ml - -
Instructions:
  1. Sterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water), solution B and solution C.

Solution A #

1500
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 0.167 1.134
2 0.50 g 0.333 1.352
3 0.20 g 0.133 1.009
4 2.00 g 1.333 -
5 5.00 g 3.333 18.503
6 3.00 g 2 14.697
7
(for liquid medium)
1000.00 ml 665.471 36939.2
8
(if required)
500.00 ml 332.736 18469.6
Instructions:
  1. Adjust pH to 4.0
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-6 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 15 g 30 -
2 500 ml 998.207 55408.8
Solution occurs in 38 other media (first defined in medium 1142 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 0.348
2 0.03 g 0.03 0.152
3 0.30 g 0.3 4.852
4 0.20 g 0.2 0.841
5 0.01 g 0.01 0.059
6 0.02 g 0.02 0.084
7 0.03 g 0.03 0.124
8 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 4.0

Associated strains

All strains for this medium: 33
Strains with modifications: 27

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar 9.993 -
Glucose 2.221 12.327
KH2PO4 1.332 9.791
Yeast extract 0.888 -
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Cultivation metadata from 33 strains BacDive

halophily slightly 3 strains
halophily not 2 strains
halophily moderate 1 strain
pH 4 - 6 5 strains
pH 2 - 4 5 strains
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