Loading ...

482. ANAEROBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.329 2.42
2 0.33 g 0.329 6.157
3 0.33 g 0.329 1.62
4 0.33 g 0.329 2.24
5 0.33 g 0.329 4.418
6 Trace element solution SL-10 1.00 ml - -
7 1.00 g 0.998 -
8
(0.1% w/v)
0.50 ml 5.0e-4 0.002
9 1.50 g 1.497 17.82
10 2.00 g 1.996 5.831
11 0.30 g 0.299 1.705
12 0.30 g 0.299 1.247
13 1000.00 ml 996.215 55298.2
Instructions:
  1. Dissolve ingredients (except bicarbonate, sucrose, cysteine and sulfide), adjust pH to 7.0, and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to pH 7.0, if necessary.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.201 55352.9
Sucrose 1.996 5.831
NaHCO3 1.497 17.82
Yeast extract 0.998 -
NH4Cl 0.329 6.157
Show more

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

Similar media

1456. DESULFOSOMA MEDIUM 79 %
872. PAPILLIBACTER MEDIUM 77 %
539. RUMINICLOSTRIDIUM CELLOBIOPARUM MEDIUM 77 %
640a. THERMOCLOSTRIDIUM CAENICOLA MEDIUM 76 %
860. ANAEROCOLUMNA MEDIUM 76 %

Latest comments

No comments for this medium.