Loading ...

500. CLOSTRIDIUM (GV) MEDIUM

Culture medium recipe

Vol.
×
  • For replace glucose with 5.00 g/l of disodium L(+)-tartrate.
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.35 g 0.345 1.982
2 0.23 g 0.227 1.667
3 0.50 g 0.493 9.218
4 2.25 g 2.219 37.968
5
(0.1% w/v in 0.1 N H2SO4)
2.00 ml 0.002 0.007
6 Trace element solution SL-10 1.00 ml - -
7 Selenite-tungstate solution 1.00 ml - -
8 1.00 g 0.986 -
9
(0.1% w/v)
0.50 ml 4.9e-4 0.002
10 3.00 g 2.959 35.218
11 0.50 g 0.493 2.001
12 0.25 g 0.247 1.677
13 , might need strain adjustment 5.00 g - -
14 Vitamin solution 10.00 ml - -
15 0.30 g 0.296 1.232
16 0.30 g 0.296 1.685
17 1000.00 ml 984.425 54643.8
18 , might need strain adjustment 2.00 g/l - -
19 , might need strain adjustment 6.00 g/l - -
20 , might need strain adjustment 5.00 g/l - -
Instructions:
  1. Dissolve ingredients (except bicarbonate, magnesium sulfate, calcium chloride, glucose, vitamins, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 7.0, dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add magnesium sulfate, calcium chloride, glucose, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2, if necessary.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 10
Strains with modifications: 10

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.228 55299
NaHCO3 2.959 35.218
NaCl 2.219 37.968
Yeast extract 0.986 -
NH4Cl 0.493 9.218
Show more

Cultivation metadata from 10 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 1 strain
phyla 3 10 strains
nutrition chemoorganotroph 1 strain
oxygen anaerobe 10 strains
Show more

Similar media

912. TREPONEMA R8 MEDIUM 84 %
120a. METHANOSARCINA BARKERI MEDIUM 83 %
487. PEPTOCLOSTRIDIUM MEDIUM (BETAINE) 82 %
963. THERMOANAEROMONAS MEDIUM 82 %
311. SPOROMUSA MEDIUM (BETAINE) 82 %

Latest comments

No comments for this medium.