DSMZ 521 . DESULFOMONILE MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 1 18.695
Na2SO4 2.0 g 2 14.081
Na2S2O3 x 5 H2O 1.0 g 1 4.029
MgSO4 x 7 H2O 1.0 g 1 4.057
CaCl2 x 2 H2O 0.1 g 0.1 0.68
KH2PO4 0.5 g 0.5 3.674
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Yeast extract 1.0 g 1 -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
NaHCO3 1.0 g 1 11.904
Vitamin solution 10.0 ml - -
Na-pyruvate 2.5 g 2.5 22.718
Na2S x 9 H2O 0.1 g 0.1 0.416
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except bicarbonate, vitamins, pyruvate and sulfide), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete the medium by adding vitamins, pyruvate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and pyruvate are sterilized by filtration. Adjust pH of the complete medium to 6.8 - 7.0.
2 Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Pyridoxine hydrochloride 5 mg 0.005 0.024
Riboflavin 5 mg 0.005 0.013
Calcium pantothenate 5 mg 0.005 0.01
p-Aminobenzoate 5 mg 0.005 0.037
Thioctic acid 5 mg 0.005 0.024
Nicotinamide 50 mg 0.05 0.409
Biotin 5 mg 0.005 0.02
Folic acid 5 mg 0.005 0.011
Vitamin B12 5 mg 0.005 0.004
Haemin
(dissolved in 0.1 N NaOH)
5 mg 0.005 0.008
1,4-Naphthoquinone
(dissolved in 0.1 N NaOH)
20 mg 0.02 0.126
Distilled water 1000 ml - -
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 173 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 04.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-pyruvate 2.5 22.718
Na2SO4 2 14.081
NH4Cl 1 18.695
NaHCO3 1 11.904
MgSO4 x 7 H2O 1 4.057
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain