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DSMZ

DSMZ 521 . DESULFOMONILE MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 521 #

ml
Equipment needed: Autoclave Filter Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 1 18.695
Na2SO4 2.0 g 2 14.081
Na2S2O3 x 5 H2O 1.0 g 1 4.029
MgSO4 x 7 H2O 1.0 g 1 4.057
CaCl2 x 2 H2O 0.1 g 0.1 0.68
KH2PO4 0.5 g 0.5 3.674
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Yeast extract 1.0 g 1 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 -
NaHCO3 1.0 g 1 11.904
Vitamin solution 10.0 ml - -
Na-pyruvate 2.5 g 2.5 22.718
Na2S x 9 H2O 0.1 g 0.1 0.416
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except bicarbonate, vitamins, pyruvate and sulfide), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete the medium by adding vitamins, pyruvate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and pyruvate are sterilized by filtration. Adjust pH of the complete medium to 6.8 - 7.0.
2 Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning.

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Pyridoxine hydrochloride 5 mg 0.005 0.024
Riboflavin 5 mg 0.005 0.013
Calcium pantothenate 5 mg 0.005 0.01
p-Aminobenzoate 5 mg 0.005 0.037
Thioctic acid 5 mg 0.005 0.024
Nicotinamide 50 mg 0.05 0.409
Biotin 5 mg 0.005 0.02
Folic acid 5 mg 0.005 0.011
Vitamin B12 5 mg 0.005 0.004
Haemin
(dissolved in 0.1 N NaOH) 		5 mg 0.005 0.008
1,4-Naphthoquinone
(dissolved in 0.1 N NaOH) 		20 mg 0.02 0.126
Distilled water 1000 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 04.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-pyruvate 2.5 22.718
Na2SO4 2 14.081
NH4Cl 1 18.695
NaHCO3 1 11.904
MgSO4 x 7 H2O 1 4.057
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain