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521. DESULFOMONILE MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.0 g 0.988 18.473
2 2.0 g 1.976 13.913
3 1.0 g 0.988 3.981
4 1.0 g 0.988 4.009
5 0.1 g 0.099 0.672
6 0.5 g 0.494 3.631
7 Trace element solution SL-10 1.0 ml - -
8 Selenite-tungstate solution 1.0 ml - -
9 1.0 g 0.988 -
10
(0.1% w/v)
0.5 ml 4.9e-4 0.002
11 1.0 g 0.988 11.763
12 Vitamin solution 10.0 ml - -
13 2.5 g 2.47 22.449
14 0.1 g 0.099 0.411
15 1000.0 ml 986.371 54751.8
16 , may stimulate growth of some strains 20.0 mg/l 0.02 -
Instructions:
  1. Dissolve ingredients (except bicarbonate, vitamins, pyruvate and sulfide), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete the medium by adding vitamins, pyruvate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and pyruvate are sterilized by filtration. Adjust pH of the complete medium to 6.8 - 7.0.
  2. Note: Prior to inoculation 10- 20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning.
Solution occurs in 1 other media (first defined in medium 521 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.005 0.024
2 5 mg 0.005 0.013
3 5 mg 0.005 0.01
4 5 mg 0.005 0.037
5 5 mg 0.005 0.024
6 50 mg 0.05 0.409
7 5 mg 0.005 0.02
8 5 mg 0.005 0.011
9 5 mg 0.005 0.003
10 5 mg 0.005 -
11
(dissolved in 0.1 N NaOH)
20 mg 0.02 0.126
12 1000 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.198 55408.3
Na-pyruvate 2.47 22.449
Na2SO4 1.976 13.913
NH4Cl 0.988 18.473
NaHCO3 0.988 11.763
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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