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58. BIFIDOBACTERIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.00 g 10.05 -
2 5.00 g 5.025 -
3 5.00 g 5.025 -
4 5.00 g 5.025 -
5 10.00 g 10.05 55.786
6 2.00 g 2.01 11.54
7 0.20 g 0.201 0.816
8 0.05 g 0.05 0.297
9 1.00 ml - -
10 5.00 g 5.025 85.984
11 0.50 g 0.503 2.861
12 Salt solution 40.00 ml - -
13
(25 mg/100ml)
4.00 ml - -
14 950.00 ml 953.062 52902.9
15 , for pH adjustment x - -
Instructions:
  1. The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave.
Solution occurs in 6 other media (first defined in medium 104 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 0.25 1.701
2 0.50 g 0.5 2.029
3 1.00 g 1 5.741
4 1.00 g 1 7.348
5 10.00 g 10 119.038
6 , might need strain adjustment 2.00 g - -
7 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave

Associated strains

All strains for this medium: 181
Strains with modifications: 180

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 993.191 55130.4
Glucose 10.05 55.786
Casein peptone, tryptic digest 10.05 -
NaCl 5.025 85.984
Yeast extract 5.025 -
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Cultivation metadata from 181 strains BacDive

halophily slightly 2 strains
halophily not 1 strain
pH 6 - 8 16 strains
pH 4 - 6 13 strains
pH 8 - 10 6 strains
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232. MRS MEDIUM WITH CYSTEINE 74 %

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