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DSMZ

DSMZ 586 . THAUERA AROMATICA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Thauera phenylacetica DSM 14743

* Modification: PH 7.3; anaerobic or aerobic. For aerobic growth, omit nitrate

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C

Main sol. 586 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 500 ml - -
Trace element solution SL-10 10 ml - -
Vitamin solution 5 ml - -
1 Adjust solutions A and B to pH 7.2, autoclave separately and combine after cooling. Add 10 ml of sterile trace elements solution SL-10 (see below) and 5 ml vitamin solution (see below).

Solution A #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.816 g 1.632 11.993
K2HPO4 5.920 g 11.84 67.977
Distilled water 500.000 ml - -

Solution B #

500
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.530 g 1.06 19.817
MgSO4 x 7 H2O 0.200 g 0.4 1.623
KNO3 2.000 g 4 39.564
CaCl2 x 2 H2O 0.025 g 0.05 0.34
Na-benzoate 0.720 g 1.44 9.993
Distilled water 500.000 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Metadata

Last modified: 23.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: * 7.3
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 11
Strains with modifications: 9
Thauera phenylacetica DSM 14743

  7.3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
K2HPO4 5.833 33.486
KNO3 1.97 19.49
KH2PO4 0.804 5.908
Na-benzoate 0.709 4.923
NH4Cl 0.522 9.762
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Cultivation metadata from 11 strains BacDive

phyla 1 11 strains
oxygen aerobe 11 strains
oxygen anaerobe 10 strains
oxygen obligate aerobe 3 strains
oxygen facultative anaerobe 1 strain
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