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592. THERMOMICROBIUM ROSEUM MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.000 g 1 -
2
(BD 211705)
1.000 g 1 -
3
(sublimed)
1.300 g 1.3 9.838
4 0.247 g 0.247 1.002
5 0.280 g 0.28 2.058
6 0.074 g 0.074 0.503
7 0.019 g 0.019 0.07
8 Trace elements 1.000 ml - -
9 999.000 ml 997.209 55353.4
10 , might need strain adjustment 20.000 g/l - -
Instructions:
  1. Adjust pH to 8.5.
  2. Add 20.0 g/l agar for solid media and pour short slants with a long butt in screw-capped tubes.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.80 g 18 90.953
2 4.40 g 44 115.389
3 0.22 g 2.2 7.651
4 0.05 g 0.5 3.279
5 0.03 g 0.3 1.24
6 0.03 g 0.3 1.507
7 100.00 ml 998.207 55408.8
Instructions:
  1. pH adjusted to 2.0 with H2SO4.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5

Associated strains

All strains for this medium: 5
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
(NH4)2SO4 1.3 9.838
Yeast extract 1 -
Tryptone 1 -
KH2PO4 0.28 2.058
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Cultivation metadata from 5 strains BacDive

halophily not 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 5 strains
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