DSMZ 603 . ANCALOMICROBIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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* Modified for Stella humosa DSM 5900

Add 0.1 g/l yeast extract.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.250 g 0.248 1.873
Glucose 0.250 g 0.248 1.374
Vitamin solution 10.000 ml - -
Hutner's salts 20.000 ml - -
Na2HPO4 0.071 g 0.07 0.495
Distilled water 980.000 ml - -
Agar , for solid medium 15.000 g/l 15 44.599
Yeast extract* 0.100 g/l - -
1 pH 7
2 Make up the medium without the vitamin solution and autoclave it. The filter-sterilized vitamin solution is added to the cooled medium. The medium may be solidified by adding 15 g/l agar.
Solution occurs in 2 other media (first defined in Medium 603).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
Biotin 2.0 mg 0.002 0.008
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Calcium pantothenate 5.0 mg 0.005 0.01
Folic acid 2.0 mg 0.002 0.005
Riboflavin 5.0 mg 0.005 0.013
Nicotinamide 5.0 mg 0.005 0.041
Distilled water 1000.0 ml - -
1 Filter-sterilized
Solution occurs in 2 other media (first defined in Medium 603).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
Solution occurs in 18 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 23.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 6
Strains with modifications: 3
Stella humosa DSM 5900

  0.1 g/l Yeast extract

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
MgSO4 x 7 H2O 0.588 2.386
(NH4)2SO4 0.248 1.873
Glucose 0.248 1.374
Nitrilotriacetic acid 0.198 1.036
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Cultivation metadata from 6 strains BacDive

pH 6 - 8 2 strains
phyla 2 5 strains
nutrition chemoorganotroph 2 strains
oxygen microaerophile 1 strain
temperature mesophilic 5 strains