Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
|FeSO4 x 7 H2O||0.20||g||0.2||0.719|
|Na2S2O3 x 5 H2O||0.08||g||0.08||0.322|
|1 Dissolve ingredients (except sucrose, sulfite and thiosulfate), adjust pH to 6.8 - 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, sulfite and thiosulfate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. The stock solution of sulfite should by freshly prepared.|
|Medium type:||Complex medium|
|Final pH:||6.8 - 7.5|
|Equipment needed:||Autoclave, Filter, Gassing station, Hungate tubes|
|Edit in Medium builder|
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
|Compound||Conc. [g/L]||Conc. [mM]|
|FeSO4 x 7 H2O||0.2||0.719|