DSMZ 61 . THERMOANAEROBACTER MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tryptone
(BD BBL)
10.00 g 10 140.687
Yeast extract 2.00 g 2 -
FeSO4 x 7 H2O 0.20 g 0.2 0.719
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Sucrose 10.00 g 10 29.214
Na2SO3 0.20 g 0.2 1.587
Na2S2O3 x 5 H2O 0.08 g 0.08 0.322
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except sucrose, sulfite and thiosulfate), adjust pH to 6.8 - 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, sulfite and thiosulfate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. The stock solution of sulfite should by freshly prepared.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8 - 7.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 17
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 10 140.687
Sucrose 10 29.214
Yeast extract 2 -
Na2SO3 0.2 1.587
FeSO4 x 7 H2O 0.2 0.719
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Cultivation metadata from 17 strains BacDive

phyla 1 16 strains
oxygen anaerobe 16 strains
temperature thermophilic 16 strains