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61. THERMOANAEROBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(BD BBL)
10.00 g 10 -
2 2.00 g 2 -
3 0.20 g 0.2 0.719
4
(0.1% w/v)
0.50 ml 5.0e-4 0.002
5 10.00 g 10 29.214
6 0.20 g 0.2 1.587
7 0.08 g 0.08 0.322
8 1000.00 ml 998.207 55408.8
10
optional
3.00 g/l 3 8.764
Instructions:
  1. Dissolve ingredients (except sucrose, sulfite and thiosulfate), adjust pH to 6.8 - 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, sulfite and thiosulfate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. The stock solution of sulfite should by freshly prepared.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 16
Strains with modifications: 16

Gas composition

  • 100 % N2

Light

20 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Sucrose 10 29.214
Tryptone 10 -
Maltose
optional
3 8.764
Yeast extract 2 -
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Cultivation metadata from 16 strains BacDive

phyla 1 16 strains
oxygen anaerobe 16 strains
temperature thermophilic 16 strains

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