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DSMZ

DSMZ 628 . MMB MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Prosthecobacter vanneervenii DSM 12252

* Modification: DSM 12252 grows on yeast extract from OXOID LP0021B or BD 212750.

Growth conditions: 3-7 days aerobic 28 °C

Main sol. 628 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.15 g 0.15 -
Yeast extract*
(Oxoid LP0021B or BD212750) 		0.15 g/l - -
Glucose 1.00 g 1 5.551
(NH4)2SO4 0.25 g 0.25 1.892
Hutner's salts 20.00 ml - -
Vitamin solution 10.00 ml - -
Distilled water 970.00 ml - -
1 pH 7.2
2 Glucose and vitamin solution should be filter-sterilized separately.

Vitamin solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.01 mg 1.0e-4 7.4e-5
Biotin 0.20 mg 0.002 0.008
Calcium pantothenate 0.50 mg 0.005 0.01
Folic acid 0.20 mg 0.002 0.005
Nicotinamide 0.50 mg 0.005 0.041
Pyridoxine hydrochloride 0.50 mg 0.005 0.024
Riboflavin 0.50 mg 0.005 0.013
Thiamine-HCl x 2 H2O 0.50 mg 0.005 0.013
Distilled water 100.00 ml - -

Hutner's salts #

1000
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 0.007
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid.
2 Adjust pH to 7.0 with KOH (~7.3 g).
3 Dissolve other salts separately and combine.
4 Adjust pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Filter
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Associated strains

All strains for this medium: 13
Strains with modifications: 2
Prosthecobacter vanneervenii DSM 12252

  0.15 g/l Yeast extract
  Yeast extract

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 1 5.551
MgSO4 x 7 H2O 0.594 2.41
(NH4)2SO4 0.25 1.892
Nitrilotriacetic acid 0.2 1.046
Peptone 0.15 -
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Cultivation metadata from 13 strains BacDive

phyla 2 13 strains
temperature mesophilic 13 strains