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683. TISSIERELLA (AMO.1) MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.80 g 5.731 98.066
2 0.40 g 0.395 1.944
3 0.30 g 0.296 3.976
4 0.15 g 0.148 1.008
5 0.27 g 0.267 4.988
6 0.20 g 0.198 1.452
7 Trace element solution SL-10 1.00 ml - -
8 Selenite-tungstate solution 1.00 ml - -
9
(OXOID)
10.00 g 9.881 -
10
(BD BBL)
0.50 g 0.494 -
11 2.00 g 1.976 18.805
12 2.00 g 1.976 16.591
13
(SIGMA)
5.64 g 5.573 48.843
14
(0.1% w/v)
0.50 ml 4.9e-4 0.002
15 4.50 g 4.447 52.932
16 0.50 g 0.494 2.813
17 Vitamin solution 10.00 ml - -
18 1000.00 ml 986.371 54751.8
19 10.00 N - -
Instructions:
  1. Dissolve ingredients (except bicarbonate, cysteine and vitamins) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 8.3 with 10 N NaOH, add bicarbonate and cysteine and dissolve. Switch gas phase to 80% N2 and 20% CO2 gas mixture and flush only the head space of the medium vessel. Dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave 15 min at 121°C. Add vitamins to the autoclaved medium from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 8.3, if necessary.
  2. Note: If creatinine is used as the substrate replacing N-methylhydantoin, prepare separately an anoxic, filter sterilized stock solution of creatinine under 100% N2 gas atmosphere.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.3
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.198 55408.3
Yeast extract 9.881 -
NaCl 5.731 98.066
N-methylhydantoin 5.573 48.843
NaHCO3 4.447 52.932
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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