DSMZ 689 . ACE MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Gassing station Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.335 g 0.335 4.494
MgCl2 x 6 H2O 6.000 g 6 29.513
MgSO4 x 7 H2O 1.000 g 1 4.057
NH4Cl 0.250 g 0.25 4.674
CaCl2 x 2 H2O 0.140 g 0.14 0.952
K2HPO4 0.140 g 0.14 0.804
NaCl 18.000 g 18 308.008
Fe(NH4)2(SO4)2 x 6 H2O 2.000 mg 0.002 -
Wolin's vitamin solution 10.000 ml - -
Yeast extract
(Difco)
1.000 g 1 -
Resazurin 1.000 mg 1.0e-3 0.004
Glucose 10.000 g 10 55.506
NaHCO3 2.000 g 2 23.808
L-Cysteine HCl x H2O 0.500 g 0.5 2.847
Distilled water 1000.000 ml - -
1 Adjust pH to 7.6.
2 Boil medium for 3 min, then cool under a stream of oxygen-free N2. Add cysteine and sodium bicarbonate. Dispense in anaerobic tubes under N2 and autoclave. A precipitate forms which will redissolve slowly over about 3 days. Before use inject separately autoclaved, anaerobic glucose solution (20% w/v). Check medium pH.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 06.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.6
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 18 308.008
Glucose 10 55.506
MgCl2 x 6 H2O 6 29.513
NaHCO3 2 23.808
MgSO4 x 7 H2O 1 4.057
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature psychrophilic 1 strain