Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
K2HPO4 | 0.23 | g | 0.23 | 1.319 | |
KH2PO4 | 0.23 | g | 0.23 | 1.688 | |
NaCl | 0.45 | g | 0.45 | 7.693 | |
(NH4)2SO4 | 0.45 | g | 0.45 | 3.402 | |
CaCl2 x 2 H2O | 0.06 | g | 0.06 | 0.408 | |
MgSO4 x 7 H2O | 0.09 | g | 0.09 | 0.365 | |
Yeast extract | 5.00 | g | 4.995 | - | |
Indigocarmine | 5.00 | mg | 0.005 | 0.011 | |
NaHCO3 | 6.40 | g | 6.394 | 76.108 | |
D-Glucose | 5.00 | g | 4.995 | 27.725 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.3 | 1.706 | |
Na2S x 9 H2O | 0.30 | g | 0.3 | 1.248 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except bicarbonate, vitamins, glucose, cysteine and sulfide), then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the bicarbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute medium under CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add vitamins, glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.8, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 06.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.7 - 6.8 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 6.394 | 76.108 |
D-Glucose | 4.995 | 27.725 |
Yeast extract | 4.995 | - |
NaCl | 0.45 | 7.693 |
(NH4)2SO4 | 0.45 | 3.402 |
Show more |