DSMZ 712 . PSEUDOBUTYRIVIBRIO MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.23 g 0.228 1.307
KH2PO4 0.23 g 0.228 1.673
NaCl 0.45 g 0.446 7.624
(NH4)2SO4 0.45 g 0.446 3.372
CaCl2 x 2 H2O 0.06 g 0.059 0.404
MgSO4 x 7 H2O 0.09 g 0.089 0.362
Yeast extract 5.00 g 4.951 -
Indigocarmine 5.00 mg 0.005 0.011
NaHCO3 6.40 g 6.337 75.43
Wolin's vitamin solution 10.00 ml - -
D-Glucose 5.00 g 4.951 27.478
L-Cysteine HCl x H2O 0.30 g 0.297 1.691
Na2S x 9 H2O 0.30 g 0.297 1.237
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, vitamins, glucose, cysteine and sulfide), then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the bicarbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute medium under CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add vitamins, glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.8, if necessary.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 06.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.7 - 6.8
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 6.337 75.43
D-Glucose 4.951 27.478
Yeast extract 4.951 -
NaCl 0.446 7.624
(NH4)2SO4 0.446 3.372
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain