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DSMZ

DSMZ 744 . HYDROGENOBACTER HALOPHILUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 744 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 2.5 g 2.5 14.353
KH2PO4 0.5 g 0.5 3.674
(NH4)2SO4 2.0 g 2 15.136
NaCl 29.3 g 29.3 501.369
MgSO4 x 7 H2O 0.2 g 0.2 0.811
CaCl2 10.0 mg 0.01 0.09
FeSO4 x 7 H2O 10.0 mg 0.01 0.036
Trace element solution 0.5 ml - -
Distilled water 1000.0 ml - -
1 Adjust pH to 6.8-7.0.
2 Incubate at 37°C under a gas phase of H2:O2:CO2 = 8:1:1.

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium 533).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O
(deionized) 		1000 ml - -
MoO3 4 mg 0.004 0.028
ZnSO4 x 7 H2O 28 mg 0.028 0.097
CuSO4 x 5 H2O 2 mg 0.002 0.008
H3BO3 4 mg 0.004 0.065
MnSO4 x 5 H2O 4 mg 0.004 0.017
CoCl2 x 6 H2O 4 mg 0.004 0.031

Metadata

Last modified: 24.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8 - 7.0
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Gas composition

  • 80 % H2
  • 10 % CO2
  • 10 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 29.3 501.369
K2HPO4 2.5 14.353
(NH4)2SO4 2 15.136
KH2PO4 0.5 3.674
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain