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DSMZ

DSMZ 770 . SULFOPHOBOCOCCUS ZILLIGII MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 770 #

ml
Equipment needed: Gassing station Serum bottle Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 970 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
Solution D 5 ml - -
Solution E 10 ml - -
1 Dissolve ingredients of solution A except carbonate, sparge with 100% N2 gas for 30 - 45 min to make it anoxic, then add carbonate and adjust pH to 9.0 - 9.3. Dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 30 ml medium per 100 ml bottle) and autoclave. Solutions B and C are autoclaved separately under 100% N2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and sterilized by filtration. To complete the medium add appropriate amounts of solutions B to E to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.5.
2 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning.
3 For transfers use 10% (v/v) inoculum.

Solution A #

970
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 32.0 mg 0.033 0.098
CaCl2 x 2 H2O 66.0 mg 0.068 0.463
MgSO4 x 7 H2O 31.0 mg 0.032 0.13
KCl 31.0 mg 0.032 0.429
ZnCl2
(0.1% w/v) 		2.1 ml 0.002 0.015
MnSO4 x H2O
(0.1% w/v) 		2.3 ml 0.002 0.014
Na2B4O7 x 10 H2O
(0.1% w/v) 		1.8 ml 0.002 -
Glycine 1.5 g 1.546 20.599
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 -
Na2CO3 230.0 mg 0.237 2.237
Distilled water 965.0 ml - -

Solution B #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1 g 100 -
Distilled water 10 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone
(BD BBL) 		1 g 100 -
Distilled water 10 ml - -

Solution D #

5
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 50 mg 10 35.969
H2SO4
(0.1 N) 		5 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Dithiothreitol
(DTT) 		150 mg 15 97.213
Distilled water 10 ml - -

Metadata

Last modified: 06.04.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glycine 1.493 19.882
Trypticase peptone 0.995 -
Yeast extract 0.995 -
Na2CO3 0.229 2.159
Dithiothreitol 0.149 0.967
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain