DSMZ 81 . MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Paracoccus denitrificans DSM 413

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 50 ml - -
Solution B 920 ml - -
Solution C 20 ml - -
Standard vitamin solution 5 ml - -
1 Solutions A, B, C are autoclaved separately for 15 min at 121°C, cooled down to 50°C and then mixed aseptically with 5.0 ml filter-sterilized standard vitamin solution (see below).The final pH of this medium should be 6.8 without adjustment.
2 For chemolithotrophic growth incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 60% H2 and 28% N2. For heterotrophic growth supplement the mineral medium with an appropriate carbon source (0.2% carbohydrate or 0.1% organic acid). For growth on nitrogen-free medium, omit NH4Cl and incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 10% H2 and 78% N2 or heterotrophically under 2% (v/v) O2 and 98% N2. For more details see Ref. 1515 and Ref. 3363.
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.3 g 46 338.022
Na2HPO4 x 2 H2O 2.9 g 58 408.569
Distilled water 50.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 1.087 20.321
MgSO4 x 7 H2O 0.50 g 0.543 2.205
CaCl2 x 2 H2O 0.01 g 0.011 0.074
MnCl2 x 4 H2O 5.00 mg 0.005 -
NaVO3 x H2O 5.00 mg 0.005 0.039
Trace element solution SL-6 5.00 ml - -
Distilled water 915.00 ml - -
Agar , if required 20.00 g 21.739 64.636
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ferric ammonium citrate 0.05 g 2.5 9.543
Distilled water 20.00 ml - -
Solution occurs in 3 other media (first defined in Medium 81).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Riboflavin 10.0 mg 0.1 0.266
Thiamine-HCl x 2 H2O 50.0 mg 0.5 1.339
Nicotinic acid 50.0 mg 0.5 4.061
Pyridoxine hydrochloride 50.0 mg 0.5 2.431
Calcium pantothenate 50.0 mg 0.5 1.049
Biotin 0.1 mg 1.0e-3 0.004
Folic acid 0.2 mg 0.002 0.005
Vitamin B12 1.0 mg 0.01 0.007
Distilled water 100.0 ml - -
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 122
Strains with modifications: 6
Paracoccus denitrificans DSM 413

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20.101 59.764
Na2HPO4 x 2 H2O 2.915 20.531
KH2PO4 2.312 16.986
NH4Cl 1.005 18.789
MgSO4 x 7 H2O 0.503 2.039
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Cultivation metadata from 122 strains BacDive

pH 6 - 8 3 strains
pH 10 - 12 2 strains
phyla 3 122 strains
nutrition chemoorganotroph 3 strains
nutrition chemolithoautotroph 3 strains
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