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833. DEHALOSPIRILLUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 878 ml - -
2 Solution B 10 ml - -
3 Solution C 10 ml - -
4 Solution D 35 ml - -
5 Solution E 20 ml - -
6 Solution F 40 ml - -
7 Solution G 1 ml - -
8 Solution H 1 ml - -
9 , might need strain adjustment 20 mg - -
Instructions:
  1. Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B and H are autoclaved separately under 100% N2 gas. Solutions C, E, F and G are prepared under 100% N2 gas and sterilized by filtration. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.3 - 7.6, if necessary.
  2. Note: For resuscitation of freeze-dried strains from ampoule, the medium must be totally reduced, i.e. resazurin has to be colorless. Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may help to reduce the medium.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.70 g 0.797 5.613
2 0.20 g 0.228 1.674
3 0.25 g 0.285 5.323
4 0.25 g 0.285 4.872
5 0.40 g 0.456 2.241
6 0.50 g 0.569 7.639
7 0.15 g 0.171 1.162
8 2.00 g 2.278 -
9 Trace element solution SL-10 1.00 ml - -
10 Selenite-tungstate solution 1.00 ml - -
11
(0.1% w/v)
0.50 ml 5.7e-4 0.002
12 875.00 ml 994.796 55219.5
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(0.1 M, pH 7.5)
10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 9 ml - -
2 Vitamin solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.75 g 50 471.752
2 35.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4.5 g 225 2044.64
2 20.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 6.4 g 160 999.775
2 40.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 25 mg 25 89.923
2
(0.1 N)
1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 50 mg 50 284.68
2 1 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 491.843 27301.4
Na2-fumarate 3.208 20.046
Na-pyruvate 2.256 20.498
Yeast extract 1.003 -
Na2CO3 0.877 8.276
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains

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