DSMZ 833 . DEHALOSPIRILLUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 883 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
Solution D 35 ml - -
Solution E 20 ml - -
Solution F 40 ml - -
Solution G 3 ml - -
Solution H 1 ml - -
1 Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B and H are autoclaved separately under 100% N2 gas. Solutions C, E, F and G are prepared under 100% N2 gas and sterilized by filtration. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.3 - 7.6, if necessary.
2 Note: Before inoculation, the medium must be completely reduced, i.e. resazurin must be colorless. The addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) can help to reduce the medium.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 0.70 g 0.792 5.575
KH2PO4 0.20 g 0.226 1.663
NH4Cl 0.25 g 0.283 5.287
NaCl 0.25 g 0.283 4.839
MgCl2 x 6 H2O 0.40 g 0.452 2.226
KCl 0.50 g 0.566 7.587
CaCl2 x 2 H2O 0.15 g 0.17 1.154
Yeast extract 2.00 g 2.262 -
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.7e-4 0.002
Distilled water 880.00 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Potassium phosphate buffer
(0.1 M, pH 7.5)
10 ml - 1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution 9 ml - -
Seven vitamins solution 1 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.75 g 50 471.752
Distilled water 35.00 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-pyruvate 4.5 g 225 2044.64
Distilled water 20.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-fumarate 6.4 g 160 999.775
Distilled water 40.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 30 mg 4.286 15.415
H2SO4
(0.1 N)
3 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 50 mg 50 284.68
Distilled water 1 ml - -
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 27.07.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3 - 7.6
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2-fumarate 6.387 39.911
Na-pyruvate 4.491 40.811
Yeast extract 1.994 -
Na2CO3 1.747 16.478
Na2SO4 0.698 4.913
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains