DSMZ 872 . PAPILLIBACTER MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 0.3 5.603
K2HPO4 0.20 g 0.2 1.147
MgCl2 x 6 H2O 0.40 g 0.4 1.966
CaCl2 x 2 H2O 0.10 g 0.1 0.68
KCl 0.50 g 0.5 6.7
NaCl 1.00 g 0.999 17.095
Yeast extract
(BD Bacto)
5.00 g 4.995 -
Trypticase peptone
(BD BBL)
5.00 g 4.995 -
Trace element solution SL-10 1.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Na2CO3 2.00 g 1.998 18.851
trans-Cinnamic acid 0.75 g 0.749 5.057
L-Cysteine HCl x H2O 0.50 g 0.5 2.844
Na2S x 9 H2O 0.50 g 0.5 2.08
Distilled water 1000.00 ml - -
1 Dissolve all ingredients except carbonate, cinnamic acid, sulfide and cysteine, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cinnamic acid, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.2 - 7.5, if necessary.
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 4.995 -
Yeast extract 4.995 -
Na2CO3 1.998 18.851
NaCl 0.999 17.095
trans-Cinnamic acid 0.749 5.057
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain