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876. DESULFOTHERMUS NAPHTHAE MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4.00 g 3.945 27.772
2 0.20 g 0.197 1.449
3 0.25 g 0.247 4.609
4 20.00 g 19.724 337.492
5 9.80 g 9.665 47.538
6 0.50 g 0.493 6.614
7 0.10 g 0.099 0.671
8 Trace element solution SL-10 1.00 ml - -
9 Selenite-tungstate solution 2.00 ml - -
10
(0.1% w/v)
0.50 ml 4.9e-4 0.002
11 1.00 g 0.986 9.305
12 0.50 g 0.493 3.57
13 0.30 g 0.296 2.687
14 Vitamin solution 9.00 ml - -
15 Vitamin solution 1.00 ml - -
16 0.40 g 0.394 1.642
17 1000.00 ml 984.425 54643.8
18 Fatty acid mixture , may stimulate growth of some strains
(1% v/v)
1.00 ml - -
Instructions:
  1. Dissolve all ingredients except carbonate, caproate, butyrate, vitamins and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add caproate, butyrate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.9, if necessary.
  2. Note: Growth can be stimulated by supplementing the medium with 1%(v/v) of fatty acid mixture (see medium 119).
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Solution occurs in 10 other media (first defined in medium 119 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 23 ml - -
2 27 ml - -
3 27 ml 22.989 225.092
4 27 ml - -
5 1000 ml 904.173 50189.2
Instructions:
  1. Adjust pH to 7.0 - 7.3 with 2 N NaOH.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.104 55403.1
NaCl 19.724 337.492
MgCl2 x 6 H2O 9.665 47.538
Na2SO4 3.945 27.772
Na2CO3 0.986 9.305
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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