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DSMZ

DSMZ 927 . HALORHABDUS UTAHENSIS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 927 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 270.00 g 270 4620.12
NaBr 0.10 g 0.1 0.972
MgSO4 x 7 H2O 20.00 g 20 81.144
KCl 5.00 g 5 67.069
NH4Cl 2.00 g 2 37.39
Tris-HCl 12.00 g 12 -
Yeast extract
(Difco) 		1.00 g 1 -
Glucose 2.00 g 2 11.101
Distilled water 1000.00 ml - -
KH2PO4 solution 5%(w/v) 2.50 ml - -
CaCl2 solution 10%(w/v) 0.50 ml - -
FeCl2/MnCl2 solution 0.25 ml - -
1 Prepare the medium, and adjust the pH to 7.6 with 5M NaOH.
2 Autoclave and when the medium has cooled add 2.5 ml of the phosphate solution, 0.5 ml of the calcium chloride solution, and 0.25 ml of the Fe/Mn solution. This strain will not grow in normal media for halobacteria, such as DSM medium 372!

Trace element solution SL-7 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		6.5 ml 1.625 44.569
FeCl2 x 7 H2O 1.5 g 1.5 11.834
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 62.0 mg 0.062 1.003
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -

KH2PO4 solution 5%(w/v) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 50 g 50 367.417
Distilled water 1000 ml - -
1 Prepare and sterilise separately

CaCl2 solution 10%(w/v) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 6 H2O 100 g 100 456.463
Distilled water 1000 ml - -
1 Prepare and sterilise separately

FeCl2/MnCl2 solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 20 g 20 100.598
MnCl2 x 4 H2O 20 g 20 101.059
Distilled water 1000 ml - -
1 Prepare and sterilise separately

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.6
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Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 270 4620.12
MgSO4 x 7 H2O 20 81.144
Tris-HCl 12 -
KCl 5 67.069
NH4Cl 2 37.39
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen aerobe 1 strain
temperature mesophilic 2 strains
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