MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

CCAP 14 . 3N-BBM+V

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C14 #

ml
Solution occurs in 1 other media (first defined in Medium C14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3
(75 g/l stock solution) 		10 ml - -
CaCl2 x 2 H2O
(2.5 g/l stock solution) 		10 ml - -
MgSO4 x 7 H2O
(7.5 g/l stock solution) 		10 ml - -
K2HPO4 x 3 H2O
(7.5 g/l stock solution) 		10 ml - -
KH2PO4
(17.5 g/l stock solution) 		10 ml 23.4 171.951
NaCl
(2.5 g/l stock solution) 		10 ml - -
Trace Elements (PIV) 6 ml - -
1 Once elements are dissolved autoclave at 15 psi for 15 minutes.
Vitamin B1 solution 1 ml - -
Vitamin B12 solution 1 ml - -
Agar , for solid medium 15 g 15 -
2 Make up to 1 litre with distilled water. For agar add 15 g per litre Bacterialogical Agar. Autoclave at 15 psi for 15 minutes.

Trace Elements (PIV) #

1000
Solution occurs in 7 other media (first defined in Medium C1).
1 Ensure elements are added in the following sequence:
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.750 g 0.75 2.231
FeCl3 x 6 H2O 0.097 g 0.097 0.359
MnCl2 x 4 H2O 0.041 g 0.041 0.207
ZnCl2 0.005 g 0.005 0.037
CoCl2 x 6 H2O 0.002 g 0.002 0.015
Na2 MoO4 x 2 H2O 0.004 g 0.004 0.017
2 Once elements are dissolved autoclave at 15 psi for 15 minutes.

Vitamin B1 solution #

100
Solution occurs in 11 other media (first defined in Medium C1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine hydrochloride
(Vitamin B1) 		0.12 g 1.2 3.558
1 Filter sterile

Vitamin B12 solution #

100
Solution occurs in 11 other media (first defined in Medium C1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine
(Vitamin B12) 		0.1 g 1 0.738
1 Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.

Metadata

Last modified: 07.10.22
Source: CCAP
Synonyms: Bold-Basal Medium with 3-fold Nitrogen and Vitamins, modified
Medium type: Defined medium
Final pH: n.d.
Edit in Medium builder

Associated strains

All strains for this medium: 782
Strains with modifications: 102

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 23.4 171.951
Agar 15 -
Na2-EDTA 0.005 0.013
Thiamine hydrochloride 0.001 0.004
Cyanocobalamine 1.0e-3 7.4e-4
Show more
CSV
JSON