CCAP 14 . 3N-BBM+V

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Solution occurs in 1 other media (first defined in Medium C14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3
(75 g/l stock solution)
10 ml - -
CaCl2 x 2 H2O
(2.5 g/l stock solution)
10 ml - -
MgSO4 x 7 H2O
(7.5 g/l stock solution)
10 ml - -
K2HPO4 x 3 H2O
(7.5 g/l stock solution)
10 ml - -
KH2PO4
(17.5 g/l stock solution)
10 ml 23.4 171.951
NaCl
(2.5 g/l stock solution)
10 ml - -
Trace Elements (PIV) 6 ml - -
1 Once elements are dissolved autoclave at 15 psi for 15 minutes.
Vitamin B1 solution 1 ml - -
Vitamin B12 solution 1 ml - -
Agar , for solid medium 15 g 15 -
2 Make up to 1 litre with distilled water. For agar add 15 g per litre Bacterialogical Agar. Autoclave at 15 psi for 15 minutes.
Solution occurs in 7 other media (first defined in Medium C1).
1 Ensure elements are added in the following sequence:
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.750 g 0.75 2.231
FeCl3 x 6 H2O 0.097 g 0.097 0.359
MnCl2 x 4 H2O 0.041 g 0.041 0.207
ZnCl2 0.005 g 0.005 0.037
CoCl2 x 6 H2O 0.002 g 0.002 0.015
Na2 MoO4 x 2 H2O 0.004 g 0.004 0.017
2 Once elements are dissolved autoclave at 15 psi for 15 minutes.
Solution occurs in 11 other media (first defined in Medium C1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine hydrochloride
(Vitamin B1)
0.12 g 1.2 3.558
1 Filter sterile
Solution occurs in 11 other media (first defined in Medium C1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine
(Vitamin B12)
0.1 g 1 0.738
1 Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.

Metadata

Last modified: 07.10.22
Source: CCAP
Synonyms: Bold-Basal Medium with 3-fold Nitrogen and Vitamins, modified
Medium type: Defined medium
Final pH: n.d.
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Associated strains

All strains for this medium: 782
Strains with modifications: 102

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 23.4 171.951
Agar 15 -
Na2-EDTA 0.005 0.013
Thiamine hydrochloride 0.001 0.004
Cyanocobalamine 1.0e-3 7.4e-4
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