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CCAP 27 . EG:JM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C27 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
EG (Euglena gracilis Medium) 500 ml - -
JM (Jaworski´s Medium) 500 ml - -
1 1:1 mixture. See separate recipes. Mix then autoclave at 15 psi for 15 minutes.

EG (Euglena gracilis Medium) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium acetate x 3 H2O 1 g 1 -
Lab Lemco powder
(Oxoid L29) 		1 g 1 -
Tryptone
(Oxoid L42) 		2 g 2 -
Yeast extract
(Oxoid L21) 		2 g 2 -
CaCl2
(1 g/l stock solution) 		10 ml - -
Deionized water 1000 ml - -
Agar , for solid medium
(Oxoid L11) 		15 g 15 -
1 Add constituents above and make up to 1 litre with deionized water. For agar add 15 g per litre Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.

JM (Jaworski´s Medium) #

1000
Solution occurs in 1 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ca(NO3)2 x 4 H2O
(4.0 g/200 ml stock solution) 		1 ml - -
KH2PO4
(2.48 g/200 ml stock solution) 		1 ml 2.34 17.195
MgSO4 x 7 H2O
(10.0 g/200 ml stock solution) 		1 ml - -
NaHCO3
(3.18 g/200 ml stock solution) 		1 ml - -
Solution 1 1 ml - -
Solution 2 1 ml - -
Solution 3 1 ml - -
NaNO3
(16.0 g/200 ml stock solution) 		1 ml - -
Na2HPO4 x 12 H2O
(7.2 g/200 ml stock solution) 		1 ml - -
Deionized water 1000 ml - -
Agar , for solid medium
(Oxoid L11) 		15 g 15 -
1 Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacterial Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.

Solution 1 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeNa-EDTA 0.45 g 2.25 -
Na2-EDTA 0.45 g 2.25 6.692

Solution 2 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 0.496 g 2.48 40.104
MnCl2 x 4 H2O 0.278 g 1.39 7.024
(NH4)6Mo7O24 x 4 H2O 0.200 g 1 0.809

Solution 3 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine 0.008 g 0.04 0.03
Thiamine HCl 0.008 g 0.04 0.119
Biotine 0.008 g 0.04 0.164

Metadata

Last modified: 07.10.22
Source: CCAP
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 39
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 7.5 -
KH2PO4 1.17 8.598
Tryptone 1 -
Yeast extract 1 -
Sodium acetate x 3 H2O 0.5 -
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