MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

CCAP 27 . EG:JM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Medium for Coelastrum sphaericum CCAP217/2

This strain uses the standard medium without modifications.

Mail sol. C27 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
EG (Euglena gracilis Medium) 500 ml - -
JM (Jaworski´s Medium) 500 ml - -
1 1:1 mixture. See separate recipes. Mix then autoclave at 15 psi for 15 minutes.

EG (Euglena gracilis Medium) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium acetate x 3 H2O 1 g 1 -
Lab Lemco powder
(Oxoid L29) 		1 g 1 -
Tryptone
(Oxoid L42) 		2 g 2 -
Yeast extract
(Oxoid L21) 		2 g 2 -
CaCl2
(1 g/l stock solution) 		10 ml - -
Deionized water 1000 ml - -
Agar , for solid medium
(Oxoid L11) 		15 g 15 -
1 Add constituents above and make up to 1 litre with deionized water. For agar add 15 g per litre Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.

JM (Jaworski´s Medium) #

1000
Solution occurs in 1 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ca(NO3)2 x 4 H2O
(4.0 g/200 ml stock solution) 		1 ml - -
KH2PO4
(2.48 g/200 ml stock solution) 		1 ml 2.34 17.195
MgSO4 x 7 H2O
(10.0 g/200 ml stock solution) 		1 ml - -
NaHCO3
(3.18 g/200 ml stock solution) 		1 ml - -
Solution 1 1 ml - -
Solution 2 1 ml - -
Solution 3 1 ml - -
NaNO3
(16.0 g/200 ml stock solution) 		1 ml - -
Na2HPO4 x 12 H2O
(7.2 g/200 ml stock solution) 		1 ml - -
Deionized water 1000 ml - -
Agar , for solid medium
(Oxoid L11) 		15 g 15 -
1 Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacterial Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.

Solution 1 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeNa-EDTA 0.45 g 2.25 -
Na2-EDTA 0.45 g 2.25 6.692

Solution 2 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 0.496 g 2.48 40.104
MnCl2 x 4 H2O 0.278 g 1.39 7.024
(NH4)6Mo7O24 x 4 H2O 0.200 g 1 0.809

Solution 3 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine 0.008 g 0.04 0.03
Thiamine HCl 0.008 g 0.04 0.119
Biotine 0.008 g 0.04 0.164

Metadata

Last modified: 07.10.22
Source: CCAP
Medium type: Complex medium
Final pH: n.d.
Edit in Medium builder

Associated strains

All strains for this medium: 39
Strains with modifications: 1
Coelastrum sphaericum CCAP217/2

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 7.5 -
KH2PO4 1.17 8.598
Tryptone 1 -
Yeast extract 1 -
Sodium acetate x 3 H2O 0.5 -
Show more
CSV
JSON