J1000
1000 .
PYVG MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1000 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase peptone
(BD-BBL) |
5.0 | g | 4.699 | - | |
|
Bacto peptone
(BD-Difco) |
5.0 | g | 4.699 | - | |
|
Yeast extract
(BD-Difco) |
10.0 | g | 9.399 | - | |
| Glucose | 10.0 | g | 9.399 | 52.168 | |
|
Resazurin
(0.05%) |
1.0 | ml | - | - | |
| Hemin solution | 10.0 | ml | - | - | |
| Hemin solution | 10.0 | ml | - | - | |
| VFA solution | 3.1 | ml | - | - | |
| Salt solution | 40.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.47 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 Prepare medium anaerobically under a 100% N2 gas-atmosphere. Dispense in anaerobic vials (1/3 vol.) and autoclave. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.20 | g | 0.2 | 1.36 |
| MgSO4 x 7 H2O | 0.48 | g | 0.48 | 1.947 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 5
other media (first defined in Medium J469).
| 1 Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml. |
Solution occurs in 2
other media (first defined in Medium J133).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Acetic acid | 17 | ml | - | - |
| Propionic acid | 6 | ml | - | - |
| n-Butyric acid | 4 | ml | - | - |
| iso-Butyric acid | 1 | ml | - | - |
| n-Valeric acid | 1 | ml | - | - |
| iso-Valeric acid | 1 | ml | - | - |
| DL-alpha-Methyl butyric acid | 1 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 9.399 | 52.168 |
| Yeast extract | 9.399 | - |
| Trypticase peptone | 4.699 | - |
| Bacto peptone | 4.699 | - |
| L-Cysteine HCl x H2O | 0.47 | - |
| Show more | ||
