J1003
1003 .
ACIDIC HB-1 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1003 #
ml
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium J744 | 1000 | ml | - | - | |
| 1 Use Medium No. 744. Before autoclaving, adjust pH of the medium to 5.5. After autoclaving, add per liter the following solutions aseptically and anaerobically from anaerobic stocks: | |||||
|
KNO3
(10%) |
20 | ml | - | - | |
|
MES
(pH 5.5, 20%) |
25 | ml | - | - | |
|
Na2S x 9 H2O
(neutralized, 3%) |
4 | ml | - | - | |
| 2 Otherwise, prepare the medium as described in the recipe of Medium No. 744. | |||||
Solution occurs in 1
other media (first defined in Medium J744).
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.000 | g | 19.066 | 326.245 | |
| MgSO4 x 7 H2O | 3.500 | g | 3.337 | 13.537 | |
| MgCl2 x 6 H2O | 2.750 | g | 2.622 | 12.895 | |
| KCl | 0.325 | g | 0.31 | 4.156 | |
| NaBr | 0.050 | g | 0.048 | 0.463 | |
| H3BO3 | 0.015 | g | 0.014 | 0.231 | |
| SrCl2 x 6 H2O | 7.500 | mg | 0.007 | 0.027 | |
| (NH4)2SO4 | 10.000 | mg | 0.01 | 0.072 | |
| KI | 0.500 | mg | 4.8e-4 | 0.002 | |
| Na2WO2 x 2 H2O | 0.100 | mg | 9.5e-5 | - | |
| CaCl2 x 2 H2O | 0.750 | g | 0.715 | 4.863 | |
| KH2PO4 | 0.500 | g | 0.477 | 3.503 | |
| NiCl2 x 6 H2O | 2.000 | mg | 0.002 | 0.015 | |
| Trace minerals (see Medium No. 151 | 10.000 | ml | - | - | |
| Resazurin | 1.000 | mg | 9.5e-4 | 0.004 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix ingredients, bring to a boil and cool under a H2-CO2 (4:1, v/v) gas mixture. Slowly add 10 ml 5% Na2S x 9H2O solution to the medium, wait for the medium to become colorless, then adjust pH to 7.0 with NaOH. Distribute medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Before inoculation, aseptically and anaerobically add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere): | |||||
|
KNO3
(10%) |
10.000 | ml | - | - | |
|
PIPES
(pH 7.0, 0.05 M) |
25.000 | ml | - | - | |
|
Na2S x 9 H2O
(neutralized, 3%) |
4.000 | ml | - | - | |
| 2 Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 5.5 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
