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JCM 1030 . DISSULFURIMICROBIUM HYDROTHERMALE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1030 #

ml
Equipment needed: Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.402 7.524
KCl 0.33 g 0.402 5.398
MgCl2 x 6 H2O 0.33 g 0.402 1.98
CaCl2 x 2 H2O 0.33 g 0.402 2.737
KH2PO4 0.33 g 0.402 2.957
Trace elements solution 10.00 ml - -
Sulfur
(powder) 		5.00 g 6.098 190.133
Distilled water 800.00 ml - -
1 Mix components, except sulfur, bring to a boil and cool down under a CO2 (100%) gas stream. While gassing, add the following components to the medium:
Trace vitamins 10.00 ml - -
NaHCO3 2.00 g 2.439 29.034
2 Bubble CO2 for 3 - 4 min and check pH to be 6.5 - 6.8. Readjust pH to the range, if necessary. Then, suspend ferrihydrite (see below) in the medium and dispense the suspension into culture vessels (e.g., 10 ml in Hungate tubes) under a CO2 gas stream. Add appropriate amount of sulfur (powder). Seal the culture vessels with butyl rubber stoppers and autoclave at 105C for 1 hr.
3 Ferrihydrite: Slowly titrate 320 ml of FeCl3 x 6H2O solution (60.0 g/L) with 10% (w/v) NaOH to pH 7.0-7.5 with agitation on a magnetic stirrer (approximately 80-100 ml NaOH solution). Remove ferrihydrite into a container, cover with water and store at room temperature overnight. Remove supernatant after centrifugation at 2000 rpm for 5 min.

Trace elements solution #

1000
Solution occurs in 3 other media (first defined in Medium J1030).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(NH4)2(SO4)2 x 6 H2O 78.40 mg 0.078 -
CoCl2 x 6 H2O 23.80 mg 0.024 0.183
NiCl2 x 6 H2O 16.60 mg 0.017 0.128
Na2MoO4 x 2 H2O 2.42 mg 0.002 0.01
Na2WO4 x 2 H2O 5.49 mg 0.005 0.017
ZnSO4 x 7 H2O 14.40 mg 0.014 0.05
CuCl2 x 2 H2O 0.17 mg 1.7e-4 1.0e-3
Na2SeO4 9.45 mg 0.009 0.05
H3BO3 0.62 mg 6.2e-4 0.01
MnCl2 x 4 H2O 19.80 mg 0.02 -
SrSO4 0.92 mg 9.2e-4 -
CrK(SO4)2 x 12 H2O 0.50 mg 5.0e-4 -
AlCl3 x 6 H2O 2.41 mg 0.002 0.01
BaCl2 2.08 mg 0.002 0.01
Na2SiO3 x 9 H2O 14.20 mg 0.014 -
KBr 11.90 mg 0.012 0.1
KI 16.60 mg 0.017 0.064
Na2SO4 71.00 mg 0.071 0.5
Distilled water 1000.00 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 6.098 190.133
NaHCO3 2.439 29.034
NH4Cl 0.402 7.524
KCl 0.402 5.398
KH2PO4 0.402 2.957
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
nutrition chemoautolithotroph 1 strain
oxygen anaerobe 1 strain
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